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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
05 JAN - 24 JAN 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Alcohols, C12-14, ethoxylated
EC Number:
500-213-3
EC Name:
Alcohols, C12-14, ethoxylated
Cas Number:
68439-50-9
IUPAC Name:
68439-50-9
Details on test material:
- Substance type: C12-C14 Fatty alkoholpolyethylenglycolether with 2 EO
- Physical state: liquid
- Analytical purity: 73% a.s. (w/w)
- Lot/batch No.: Pt. 24, Tank 7

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): Stock solution (0.23 g substance in 20 g quarz sand) was mixed with 1680 g steril substrate to a concentration of 100 mg/kg dry substrate.

Test organisms

open allclose all
Species:
Triticum aestivum
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: wheat
- Plant family: Poacea
Species:
Brassica alba
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: White mustard
- Plant family: Brassicaceae
Species:
Lepidum sativum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: Garden cress
- Plant family: Brassicaceae

Study design

Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
yes
Total exposure duration:
19 d

Test conditions

Test temperature:
20 - 24 °C
pH:
5.0 - 7.5
Moisture:
approximately 60%
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): plastic pots, diameter 6 cm (bottom) and 8 cm (top), 6 cm (height)
- Amount of soil: approximately 100 g substrate
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA soil Speyer (Sp. 2.3)
- Soil texture (if natural soil): particle < 20 µm: 10 - 20%
- Organic carbon (%): < 1.5%



GROWTH CONDITIONS
- Photoperiod: 16 h day / 8 h night
- Light intensity and quality: day: 1500 - 2000 lux, night: approximately 50 lux
- Day/night temperatures: 20 - 24 °C
- Relative humidity (%): 90% - 100%
- Interval of applications: 1 - 2 times per week

Nominal and measured concentrations:
100 mg/kg soil
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Species:
Triticum aestivum
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Species:
Brassica alba
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Species:
Lepidum sativum
Duration:
19 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Remarks:
and growth
Details on results:

SEEDLING EMERGENCE
- Percent seedling emergence: Triticum aestivum: 100 mg/kg: 90% (85% control), Brassica alba: 100 mg/kg: 80% (80% control), Lepidum sativum: 100 mg/kg: 95% (control: 95%)
- Fresh weight: Triticum aestivum: 100 mg/kg: 357 mg (389 mg control), Brassica alba: 100 mg/kg: 248 mg (228 mg control), Lepidum sativum: 100 mg/kg: 71 mg (control: 61 mg)

Applicant's summary and conclusion