Reaction mass of sodium 1-methoxy-1-oxohexadecane-2-sulphonate and sodium methyl 2-sulphooctadecanoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

The test has been conducted according to OECD guidelines. Not GLP. No measurements of test concentrations or dissolved oxygen were performed. The data in the publication does not give sufficient experimental details to derive the LC50's.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Principles of method if other than guideline:

No measurements of concentrations were performed. No measurement of dissolved oxygen.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia Magna
- Strain: supplied by the Independent Administrative Institution, Chemicals Evaluation and Research Institute (CERI, Japan)
- Source: Chemicals Evaluation and Research Institute (CERI, Japan)
- Age at study initiation (mean and range, SD): within 24 h of birth
- Method of breeding: passage breeding in the laboratory
- Feeding during test: None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None.

Test temperature:

20 ± 1 ºC

Salinity:

Not applicable.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL test solution in a 100 mL glass beaker
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:


TEST MEDIUM / WATER PARAMETERS
Dechlorinated tap water


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h on, 8h off
- Light intensity: room light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- 0, 5.6, 10, 18, 32, 56, 100 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The 48hr EC50 was determined to be 17.2 mg/L (nominal)

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 12, 2000 - December 10, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The concentration of the study substance in the study solution was measured at the beginning of the study and at 48 hours. From each container, approx. 1.5 mL of the study solution was taken and collected in a vial. Methanol was added in an equal amount, and subsequestly mounted on an LC-MS autosampler.

Vehicle:

no

Details on test solutions:

A solution containing 300 mg/L of study substance was added to dilution water (M4 medium) to prepare study solutions of the pre-determined concentrations in the required amount.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: juveniles from parents that have been acclimatized for 19 days
- Source: National Institute for Environmental Studies
- Age at study initiation (mean and range, SD): 24 hr
- Method of breeding: captive breeding at the TRC Research Division
- Feeding during test: no feeding


ACCLIMATION for the parents
- Acclimation period: 19 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: 0.1-0.2 mg C/day per daphnid; chlorella vulgaris

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None.

Hardness:

252 mg CaCO3/L

Test temperature:

20.2-20.7 ºC

pH:

7.2-7.6

Dissolved oxygen:

8.2-8.8 mg/L

Salinity:

Not applicable.

Nominal and measured concentrations:

The initial study substance concentration was 0.4-3.9 mg/L (concentrations pre-set at 0.48-5 mg/L), and the ratio of the measurements to a set concentration was 74.7-84.5%. The concentration at 48 hours was 0.4-3.7 mg/L (concentrations pre-set at 0.48-5 mg/L) with a ratio of 66.9-75%. Because the actual measurements were outside of ± 20% of the set concentrations, geometric mean values were used to calculate the test results.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass with a capacity of 100 mL
- Aeration:
- No. of organisms per vessel: 5 daphnids/concentration
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate:


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium(OECD 211)



OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hour light, 8 hours dark
- Light intensity: room light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 (nominal conc: 0.48, 0.86, 1.54, 2.78 and 5 mg/L)
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: not indicated
- Results used to determine the conditions for the definitive study: Median Immobilization Concentration 48hrs was 5 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.24 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.63 - 2.21 mg/L

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

2.02 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 1.66-2.59 mg/l

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.63 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2.21 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

None.

Results with reference substance (positive control):

In the acute immobilization test of the reference substance, an 48hr EC50 of 0.9 mg/L was found.

Reported statistics and error estimates:

Immobilization ratio (%) and median Immobilization Concentration was calculated by using TOXDAT MULTI-METHOD PROGRAM Binominal method developed by EPA

Validity criteria fulfilled:

yes

Conclusions:

EC50 48hr = 1.24 mg/L (0.63-2.21 mg/L 95% CI)

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

The endpoint of short-term toxicity to aquatic invertebrates of the target substance, reaction mass of sodium methyl 2-sulphooctadecanoate and sodium 1-methoxy-1-oxohexadecane-2-sulphonate, was based on the data from the two main components, C16MES and C18MES, and substances similar to the target substance as source chemicals. No definitive value for the reaction mass was derived nor necessary as the data of the two components of the reaction mass are used in separate environmental risk assessments.
Please see the read-across justification in section 13.2

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.24 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

Substance: C16MES (223-676-0)

Basis for effect:

mobility

Remarks on result:

other: 0.63-2.21 mg/L

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.252 - < 0.373 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Remarks:

Substance: C18MES (223-770-1)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Substance: C14MES (223-675-5)

Basis for effect:

mobility

Conclusions:

The endpoint of short-term toxicity to aquatic invertebrates of the target substance, reaction mass of sodium methyl 2-sulphooctadecanoate and sodium 1-methoxy-1-oxohexadecane-2-sulphonate, was based on the data from the two main components, C16MES and C18MES, and substances similar to the target substance as source chemicals. No definitive value for the reaction mass was derived nor necessary as the data of the two components of the reaction mass are used in separate environmental risk assessments.

Description of key information

C16MES: EC50 48hr = 1.24 mg/L (0.63-2.21 mg/L 95% CI) (geom. mean of measured values)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.24 mg/L

Additional information

Supporting data from similar substance with shorter chain length:

C14MES: EC50 48hr = 17.2 mg/L (nominal)

The combination toxicology cannot be used to determine one single key value for chemical safety assessment of the reaction mass - indeed, the proportion of both components that enter the environment (after the STP) is not deemed to be the same as the proportion of the 2 components before the STP