4-[difluoro(3,4,5-trifluorophenoxy)methyl]-2',3,5-trifluoro-4''-pentyl-1,1':4',1''-terphenyl

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Principles of method if other than guideline:

Solubiliser was applied to achieve the final concentrations above the water solublity limit.

GLP compliance:

yes

Radiolabelling:

no

Vehicle:

yes

Remarks:

HCO-40

Details on preparation of test solutions, spiked fish food or sediment:

HCO-40 was used as solubiliser.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
Length at study initiation: 8.3 cm
- Weight at study initiation: 6.4 g

Route of exposure:

aqueous

Justification for method:

other: most valid guideline in 2008

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

28 d

Test temperature:

25 ± 2 °C

Details on test conditions:

According to Guideline OECD 305 (test year 2008)
RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations : LC50 (96-h) > 100 mg/L, Rice fish (Oryzias latipes)

After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 4, 7, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.5 and 0.05 mg/L were maintained at about 100 % using solubiliser, respectively. At days 4, 7, 14, 21 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted using organic solvents. The extract was analysed with HPLC. Due to the low bioaccumulation potential, no depuration phase was required.

Nominal and measured concentrations:

- Nominal concentrations 1st Concentration area 0.001 mg/L and 2nd Concentration area 0.0001 mg/L
nominal concentration 0.001 mg/L: HCO-40: 1 mg/L
analytically measured concentrations:
day 4: 0.000991 mg/L
day 7: 0.00100 mg/L
day 14: 0.00102 mg/L
day 21: 0.000994 mg/L
day 28: 0.000994 mg/L

nominal concentration 0.0001 mg/L, HCO-40: 0.1 mg/L
analytically measured concentrations:
day 4: 0.000101 mg/L
day 7: 0.0000999 mg/L
day 14: 0.000100 mg/L
day 21: 0.000100 mg/L
day 28: 0.000100 mg/L

Lipid content:

ca. 5 %

Time point:

other: literature value

Remarks on result:

other: E. G. Bligh and W. J. Dyer, Can. J. Biochem, Physiol., 37, 911 (1959)

Key result

Conc. / dose:

0.001 mg/L

Type:

BCF

Value:

63 dimensionless

Key result

Conc. / dose:

0 mg/L

Type:

BCF

Value:

53 dimensionless

Details on kinetic parameters:

Steady state was reached within 14 days.
Due to the low uptake, no depuration phase was necessary.

Details on results:

- Mortality of test organisms: no effects
- Behavioural abnormalities: no effects
- Observations on body length and weight: no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects

Results

No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 63 and 53 (mean: 58). Due to the low bioaccumulation potential, no depuration phase was required.

Conclusion

The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 63 and 53 (mean 58).

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no relevant bioaccumulation at concentration levels at or above the water solubility. The steady state BCF values were at 63 and 53 (mean 58).

Executive summary:

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the test material of 0.001 and 0.0001 mg/L were used in this study. HCO-40 was used as vehicle. The exposure was maintained during the course of the study at about 100 % of the nominal concentrations. The concentrations were verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 63 and 53 (mean: 58). Due to the low bioaccumulation potential, no depuration phase was required. The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 63 and 53 (mean 58).

Reference 2

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2007-2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

For this endpoint information from structural similar compounds is available. The studies for these similar compounds were performed according to GLP and the methods applied are fully compliant with OECD TG 305. See chapter 13 report for a more detailed justification.

Reason / purpose for cross-reference:

read-across source

GLP compliance:

yes

Key result

Conc. / dose:

0.001 mg/L

Type:

BAF

Value:

63 dimensionless

Key result

Conc. / dose:

0 mg/L

Type:

BCF

Value:

53 dimensionless

Description of key information

The source material 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-2',3,5-trifluoro-4''-propyl-1,1':4',1''-terphenyl showed no relevant bioaccumulation at concentration levels at or above the water solubility. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 305. The steady state BCF values were at 63 and 53 (mean 58) (reference 5.3.1-2).

Key value for chemical safety assessment

BCF (aquatic species):

58 dimensionless

Additional information

A read-across approach to the structural analogue 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-2',3,5-trifluoro-4''-propyl-1,1':4',1''-terphenyl, CAS 303186-36-9 was applied for the endpoint "Bioaccumulation" (reference 5.3.1-1).

The purpose of the study was to determine the bioconcentration potential of the source material in fish under flow-through conditions. The study was performed according to the OECD Guideline for Testing of Chemicals 305 (Bioconcentration: Flow-through Fish Test) adopted on June 14, 1996 using two test material concentrations for exposure to Cyprinus carpio. Two environmental concentrations of the source material of 0.001 and 0.0001 mg/L were used in this study. HCO-40 was used as vehicle. The exposure was maintained during the course of the study at about 100 % of the nominal concentrations. The concentrations were verified analytically using a HPLC method with UV detection. No signs of toxicity were observed throughout the study. The analysis revealed steady state BCFs of 63 and 53 (mean: 58). Due to the low bioaccumulation potential, no depuration phase was required. The source test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 63 and 53 (mean 58). It is assumed that the target substance would as well have low bioaccumulation potential due to the high similarity in the structures (reference 5.3.1-2).