N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]

Vapour pressure

Link to relevant study record(s)

Reference

Endpoint:

vapour pressure

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-11-20 to 2018-01-31

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 104 (Vapour Pressure Curve)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.4 (Vapour Pressure)

Deviations:

no

GLP compliance:

no

Type of method:

effusion method: vapour pressure balance

Key result

Temp.:

20 °C

Vapour pressure:

0 Pa

Key result

Temp.:

25 °C

Vapour pressure:

0 Pa

Key result

Temp.:

50 °C

Vapour pressure:

0 Pa

The vapour pressure was measured in the temperature range of 65 °C to 110 °C. The measured vapour pressures at the corresponding temperatures are listed in the table below. The mass of test item was determined (with Sartorius R200D) before and after the vapor pressure measurement. A mass loss of approx. 2 % (w/w) was observed.

Temperature / °C	Vapour pressure / hPa
65	7.2 × 10E-5
70	4.5 × 10E-5
80	1.2 × 10E-5
90	4.3 × 10E-6
100	2.9 × 10E-6
110	5.7 × 10E-6

The data points measured at 65 °C, 70 °C and 80 °C showed a typical deviation possibly caused by volatile impurities at the beginning of a measurement. The test item showed an unusual vapor pressure behaviour during the measurement. All other values were below the detection limit (1 × 10E-5 hPa). The unusual behaviour did not influence the final results.

The values for 20 °C, 25 °C and 50 °C were estimated according to the Antoine equation.

Conclusions:

The vapour pressure of the test item was determined according to the OECD guidline 104 by effusion method using a vapour pressure balance. The vapour pressure was measured in the temperature range of 65 °C to 110 °C. However, the measured values were close to the detection limit and therefore the vapour pressures for 20 °C, 25 °C and 50 °C were estimated using Antoine equation:
20 °C: < 9.9 x 10E-8 Pa;
25 °C: < 1.9 x 10E-7 Pa;
50 °C: < 3.8 x 10E-6 Pa.

Executive summary:

The vapour pressure of the test item was determined according to the OECD guideline 104 by effusion method using a vapour pressure balance. The vapour pressure was measured in the temperature range of 65 °C to 110 °C. During the measurement the test item showed an unusual vapor pressure behaviour caused by volatile impurities (please refer to results in section "any other information on result incl. tables"). However, the values were in the range of the detection limit and therefore, the values for 20 °C, 25 °C and 50 °C were estimated using Antoine equation.The unusual behaviour did not influence the final results:

20 °C: < 9.9 x 10E-8 Pa;

25 °C: < 1.9 x 10E-7 Pa;

50 °C: < 3.8 x 10E-6 Pa.

Description of key information

The vapour pressure of the test item was determined according to the OECD guideline 104 by effusion method using a vapour pressure balance. The vapour pressure was measured in the temperature range of 65 °C to 110 °C. During the measurement, the test item showed an unusual vapor pressure behaviour caused by volatile impurities (please refer to results in section "any other information on result incl. tables"). However, the values were in the range of the detection limit and therefore, the values for 20 °C, 25 °C and 50 °C were estimated using Antoine equation. The unusual behaviour did not influence the final results:

20 °C: < 9.9 x 10E-8 Pa;

25 °C: < 1.9 x 10E-7 Pa;

50 °C: < 3.8 x 10E-6 Pa.

Key value for chemical safety assessment

Vapour pressure:

0 Pa

at the temperature of:

25 °C