Registration Dossier
Registration Dossier
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EC number: 940-820-4 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Under the test conditions the percentage biodegradation of EMI-DBS reached a mean of 32.1% after 28 days based on the measured COD of the test item. Therefore the test item can be considered to be not ready biodegradable. Based on biodegradation being recorded as > 20% the study provides evidence of the test substance being inherently biodegradable (as per ECHA's Guidance on information requirements and chemical safety assessment, Chapter R.7b: Endpoint specific guidance, section R.7.9.4.1 Laboratory data on degradation/biodegradation).
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
A study was conducted to determine the biodegradation of the registered substance in water. The biodegradation of the test substance in water was determined in accordance with the OECD Guideline for Testing of Chemicals 301D. The ready biodegradability of the substance was determined in a closed bottle test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The test system was a microbial inoculum of good quality, collected from the sewage plant for domestic sewage. The sludge was washed by centrifugation filtered through cotton wool and aerated until use. Based on the determined dry weight content, the washed sludge suspecsion contained 4 g dry material per litre. Under the test conditions the percentage biodegradation of EMI-DBS reached a mean of 32.1% after 28 days based on the measured COD of the test item. Therefore the test item can be considered to be not ready biodegradable. Based on biodegradation being recorded as > 20% the study provides evidence of the test substance being inherently biodegradable (as per ECHA's Guidance on information requirements and chemical safety assessment, Chapter R.7b: Endpoint specific guidance, section R.7.9.4.1 Laboratory data on degradation/biodegradation). According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 2.7 mg/l on the activated sludge microorganisms because degradation was >25% within 14 days.
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