Registration Dossier
Registration Dossier
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EC number: 942-324-3 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 162691-58-9
- EC Number:
- 605-300-0
- Cas Number:
- 162691-58-9
- IUPAC Name:
- 162691-58-9
- Details on test material:
- - Name of test material (as cited in study report): C9-Acetal
- Physical state: liquid
TOC: 631 mg/g
DOC: 629 mg/g
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- 30 mg/L (dry matter content) from laboratory wastewater treatment plants with municipal and synthetic sewage.
- Duration of test (contact time):
- 22 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 28.2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: inorganic medium according to guideline
- Test temperature: ambient
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Shaken flasks cultured under aerobic conditions and constant temperature
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: continuous aeration as prescribed by the guideline
- Measuring equipment: DOC analyser (Shimadzu TOC 500 and TOC 5000)
- Test performed in open system: yes
SAMPLING
- Sampling frequency: day 0, 1, 3, 7, 10, 14, 18, 21, 22
CONTROL AND BLANK SYSTEM
- Inoculum blank: BC, 2 replicates
- Abiotic sterile control: PC, 1 replicate
- Toxicity control: RS, 1 replicate
- Other: inhibition control (IH, 1 replicate); adsorption control (AC, 1 replicate)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- - Physical-chemical elimination after 22-days: 3% of DOC
- Elimination through adsorption after 22 days: 9% of DOC
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 91
- Sampling time:
- 22 d
- Details on results:
- - Degradation of >70 % was observed after 7 days. The 10-day window criterion was met.
- Adaptation phase: 7 days.
- Degradation phase: 3 days.
Applicant's summary and conclusion
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