Registration Dossier
Registration Dossier
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EC number: 943-540-0 | CAS number: -
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The effect of the test item on the respiration of activated sewage sludge was tested using OECD Guideline 209 and gave a 3-hour EC50 value of 340 mg/L and an EC10 value of 74 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was < 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 340 mg/L
- EC10 or NOEC for microorganisms:
- 74 mg/L
Additional information
TEST GUIDELINE
A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method was designed to be compatible with OECD Guidelines for Testing of Chemicals (2010) No 209 "Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation).
METHODS
Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 100, 180, 320 and 560 mg/L (5 replicates) for a period of 3 hours at temperatures of between 20 and 22°C with the addition of a synthetic sewage as a respiratory substrate.
At the request off the Sponsor, an additional concentration was employed in order to determine the No Observed Effect Concentration (NOEC).
The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item (3,5 -dichlorophenol).
RESULTS
The effect of the test item on the respiration of activated sewage sludge gave a 3 -hour EC50 value of 340 mg/L and a 3 -hour EC10 value of 74 mg/L. The No Observed Effect Concentration (NOEC) after 3 hours exposure was less than 100 mg/L. A NOEC was not achieved during the definitive test. The reference item gave a 3 -hour EC50 value of 6.8 mg/L (95 % confidence limits 5.3 to 8.8 mg/L).
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