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EC number: - | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The 72-hour EL50, ErL50 and EyL50 values for cell density, growth rate and yield were determined to be 37, 40, and 28 mg/L, respectively. The 72-hour NOEL values for cell density, growth rate and yield were determined to be 6.1 mg/L, for all three endpoints, and were determined based on evaluation of the dose-response. The introduction of test item to the test medium reduced the pH substantially in the nominal 63 and 200 mg/L treatment groups. Adverse effects observed in these treatments may have been caused by the decline in pH, however, since the addition of the test substance caused the decline in pH they were considered to be treatment related effects (OECD 201, EU Method C.3 and ASTM E1218 -04).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 40 mg/L
- EC10 or NOEC for freshwater algae:
- 6.1 mg/L
Additional information
GUIDELINE
The study protocol was based on procedures outlined in Official Journal of the European Communities No. L383, Method C.3: AlgalInhibition Test; OECD Guideline for Testing of Chemicals, 201: Freshwater Alga and Cyanobacteria,Growth Inhibition Test; and in ASTM Standard E1218-04 Standard Guide for Conducting Static Toxicity Tests with Microalgae.
METHODS
The freshwater alga, Raphidocelis subcapitata, was exposed to a geometric series of five nominal WAF loading rates of test item ranging from 1.9 to 200 mg/L. The toxicity of the test item to R. subcapitata was assessed based on effects on cell density, growth rate and yield relative to the negative control.
RESULTS
The 72-hour EL50, ErL50 and EyL50 values for cell density, growth rate and yield were determined to be 37, 40, and 28 mg/L, respectively. The 72-hour NOEL values for cell density, growth rate and yield were determined to be 6.1 mg/L, for all three endpoints, and were determined based on evaluation of the dose-response. The introduction of test item to the test medium reduced the pH substantially in the nominal 63 and 200 mg/L treatment groups. Adverse effects observed in these treatments may have been caused by the decline in pH, however, since the addition of the test substance caused the decline in pH they were considered to be treatment related effects.
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