reaction mass of (9Z,11E)-octadeca-9,11-dienoic acid and (10E,12Z)-octadeca-10,12-dienoic acid and octadec-9-enoic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Oleic acid

EC Number:

204-007-1

EC Name:

Oleic acid

Cas Number:

112-80-1

Molecular formula:

C18H34O2

IUPAC Name:

octadec-9-enoic acid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Source of Inoculum: activated sludge from a STP effluent from the STP Hochdahl (1 ml/L culture solution), with primarliy munucipal sewage effluent.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2 - 5 mg/L

Based on:

act. ingr.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD 301 D
- Additional substrate:
- Solubilising agent (type and concentration if used): Disponil NP 9.5EO 5PO
- Test temperature: troom temperature

- pH: not indicated
- pH adjusted: not indicated
- Suspended solids concentration:


TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 2 per concentration
- Method used to create aerobic conditions: stirring
- Test performed in open system: no


SAMPLING
- Sampling frequency: after 0, 7, 21, 28 d
- sampling storage before analysis: no

CONTROL AND BLANK SYSTEM
- Inoculum blank: 4
- Solvent control: 2 per concentration
- Reference substance: 2

STATISTICAL METHODS: not applicable

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

< 0

Sampling time:

0 d

Remarks on result:

other:

Remarks:

Based on test 2 mg/L AS

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

70 - 80

Sampling time:

7 d

Remarks on result:

other:

Remarks:

Based on test 2 mg/L AS

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

80 - 90

Sampling time:

21 d

Remarks on result:

other:

Remarks:

Based on test 2 mg/L AS

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

90 - 100

Sampling time:

28 d

Remarks on result:

other:

Remarks:

Based on test 2 mg/L AS

Details on results:

degradation profile of 5 mg/L AS:
day 7: 60 - 70 %
Day 21: 60 - 70 %
day 28: 60 - 70 %

BOD5 / COD results

Results with reference substance:

sodium benzoate:
day 7: 70 - 80 %
day 21: 90 - 100 %
day 28: 90 - 100 %