Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 946-846-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Octadeca-dienoic acid (3-dimethylamino-propyl)-amide
- Molecular formula:
- C23H44N2O
- IUPAC Name:
- Octadeca-dienoic acid (3-dimethylamino-propyl)-amide
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- DOC at test start
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Origin: All fish used in the test originated from an own breeding (hatching date: December 02, 2002). Parental fish were supplied by AQUARIUM AM AEGI, Feldstr. 7, D-30159 Hannover.
Holding: Holding was performed at the test facility at 23 ± 2 °C and diffuse light (0.1-10 µmol/m2s, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value. Only fish from a population with mortality < 5 % within the last 7 days before start of the study were used in the test. No disease treatments were administered throughout holding and testing.
Water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
Nominal water parameters:
Total hardness: 10 - 250 mg CaCO3/L
pH-value: 6.0 - 8.5
The water is analysed once a year acc. to German tap water regulation.
Feeding: Food was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day. The test fish were not fed 24 h before the test was started.
Food: Trouvit 40 I 2; MILKIVIT, D-86666 Borgheim
Number of test fish: 7 zebrafish were used per test concentration and control.
Loading, length of fish: Fish density in the tanks was less than 1 g fish per liter test solution. Average body length at the test start: 2.49 cm
Average body weight at the test start: 0.15 g
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 67 mg/L as CaCO3
- Test temperature:
- 23 ± 2 °C
- pH:
- 7.11 - 7.69
- Dissolved oxygen:
- 95-99% saturation
- Nominal and measured concentrations:
- Test concentrations: 62.5- 125- 250- 500 - 1000 µg/L, prepared with dilution water (nominal)
Stock solution: A stock solution of 10 mg/L was prepared - Details on test conditions:
- A static test was performed with one application at the test initiation.
Test duration: 96h
Test vessel: Glass-aquaria loosely covered by glass tops were used.
Test volume: The test volume was about 4 L per vessel.
Aeration: Gentle aeration was provided.
Dilution water: Same as for holding.
Replicates: One replicate per test concentration and control.
Introduction of fish: Fish were introduced randomly to individual replicates.
Feeding of test fish: The fish were not fed during the test.
Water temperature: 23 ± 2 °C
Dissolved oxygen concentration: Not less than 60 % of air saturation value.
Light intensity: 0.1- 10 µmol/m2· s
Photoperiod: A natural photoperiod occurred during the test.
Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 354 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 250 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 500 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- After 24, 48, 72 and 96 h mortality and behaviour of the test fish was observed.
The test item was clearly dissolved throughout the test.
No symptoms of toxicity were seen up to 250 µg/l. At 500 µg/l one fish showed lethargy before mortality occured.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test substance was found to be toxic to zebrafish after 96 h at the nominal concentration level of 500 µg/L and higher.
The LC50 (96 h) was 354 µg/L (330- 379 µg/L), the NOEC after 96 h was the test item concentration 250 µg/L (all nominal concentrations). - Executive summary:
The acute toxicity of the test item to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) from 2003-03-31 to 2003-04-04 at DR.U.NOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE, 31157 Sarstedt, Germany.
A static test was conducted with the nominal concentrations 62.5 - 125 - 250 - 500 - 1000 µg/L.
Duration of the test was 96 h. 7 test organisms were exposed to each test concentration and control.
All effect values are based on the nominal concentration of the test item.
The stock solution of the substance was analytically verified via analysis of dissolved organic carbon (DOC, according to DIN guideline 38409 part 3) at test start.
The test substance was found to be toxic to zebrafish after 96 h at the nominal concentration level of 500 µg/L and higher.
The LC50 (96 h) was 354 µg/L (330- 379 µg/L), the NOEC after 96 h was the test item concentration 250 µg/L (all nominal concentrations).
Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits.
The validity criteria of the guideline were met.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.