Reaction mass of 1,4-bis(methoxymethyl)benzene, 1,6-dihydroxynaphtalene and Epichlorohydrin

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 21 February 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test temperature:

Culture temperature was checked at the beginning of the experiment and each day thereafter in a flask filled with water, in the climatic chamber. In addition, water temperature was continuously measured (with a min/max thermometer) within the climate chamber.

pH:

The range of the pH was 7.74 – 8.97 during the experiment.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

The ErC50 is >100 mg/l based on nominal loading rates.

Executive summary:

The effect of CAS 577978-76-8 test item was assessed on algal growth using the unicellular green alga Pseudokirchneriella subcapitata, over an exposure period of 72 hours.

Because a significant inhibition was not observed during the preliminary concentration range-finding test, a Limit Test was carried out using only one test concentration at 100.0 mg/L nominal loading rate and one control group.

The test concentration was analytically determined at the start and at the end of the experiment, but the test item concentration could not be analytically quantified during the experiment, consequently, the measured concentrations were below the Limit of Quantification (LOQ = 0.484 mg/L). The biological results are based on nominal loading rate.

The test design included six replicates at test concentration groups and six replicates for the untreated control.

Statistical comparisons of biomass, average specific growth rates and yield in control and in treated groups were carried out using 2 Sample t-Test (α = 0.05) by TOXSTAT software.

The ErL50, EbL50 and EyL50 values of the test item were determined directly from the raw data.

The ErC50 is >100 mg/l.

Description of key information

The effect of CAS 577978-76-8 test item was assessed on algal growth using the unicellular green alga Pseudokirchneriella subcapitata, over an exposure period of 72 hours.

Because a significant inhibition was not observed during the preliminary concentration range-finding test, a Limit Test was carried out using only one test concentration at 100.0 mg/L nominal loading rate and one control group.

The test concentration was analytically determined at the start and at the end of the experiment, but the test item concentration could not be analytically quantified during the experiment, consequently, the measured concentrations were below the Limit of Quantification (LOQ = 0.484 mg/L). The biological results are based on nominal loading rate.

The test design included six replicates at test concentration groups and six replicates for the untreated control.

Statistical comparisons of biomass, average specific growth rates and yield in control and in treated groups were carried out using 2 Sample t-Test (α = 0.05) by TOXSTAT software.

The ErL50, EbL50 and EyL50 values of the test item were determined directly from the raw data.

The ErC50 is >100 mg/l.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

EC10 or NOEC for freshwater algae:

100 mg/L