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Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.4150 (Terrestrial Plant Toxicity Tier I (vegetative vigor))
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3S,6S,7R,8R)-8-benzyl-3-(3-hydroxy-4-methoxypyridine-2-amido)-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl 2-methylpropanoate
Cas Number:
167173-85-5
Molecular formula:
C26H30N2O9
IUPAC Name:
(3S,6S,7R,8R)-8-benzyl-3-(3-hydroxy-4-methoxypyridine-2-amido)-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl 2-methylpropanoate
Specific details on test material used for the study:
Substance name: X642188
Lot#: XS9-129333-47
Purity: 100%

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes

Test organisms

open allclose all
Species:
Lolium perenne
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Marabella
Common name: Ryegrass
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Tamara
Common name: Onion
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Primus
Common name: Oilseed rape
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Demetra
Common name: Soybean
Species:
Daucus carota
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Napoli
Common name: Carrot
Species:
Cucumis sativus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Profi
Common name: Cucumber
Species:
Beta vulgaris
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Wiebke
Common name: Sugar beet
Species:
Helianthus annuus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Extrasol
Common name: Sunflower
Species:
Lycopersicon esculentum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Variety: Golden Pearl
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
Variety: Aragon

Study design

Test type:
vegetative vigour test
Study type:
other: greenhouse
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
17.5 °C and 25.2 °C
pH:
7.5
Nominal and measured concentrations:
Controls (Deionized water and PRESTO adjuvant), 32.5, 65, 130, 260 g a.i./ha

Results and discussion

Effect concentrationsopen allclose all
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
130 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Biomass (fresh weight)
Remarks on result:
other: Sugar beet showed significant fresh weight reduction of around 14% after application of 260.0 g/ha.
Key result
Species:
other: Glycine max and Beta vulgaris
Duration:
21 d
Basis for effect:
phytotoxicity
Remarks on result:
other: The tested plant species showed no phytotoxic symptoms except soybean and sugar beet. Very slight chlorosis and deformation were observed in soybean in all treatments treated with test substance. Sugar beet showed slight stunting after application of 260.
Key result
Species:
Avena sativa
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Lolium perenne
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Brassica napus
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Daucus carota
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Cucumis sativus
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Beta vulgaris
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested
Key result
Species:
Lycopersicon esculentum
Duration:
21 d
Dose descriptor:
other: NOER
Effect conc.:
>= 260 g/ha
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: Plant survival
Remarks on result:
other: Highest dose tested

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Plant survival: 21-d NOER (all species tested): >= 260 g a.i./ha (highest dose tested)
Phytotoxicity: Very slight chlorosis and deformation were observed in soybean in all treatments treated with test substance. Sugar beet showed slight stunting after application of 260.0 g a.i./ha.
Biomass: 21-d NOER (Beta vulgaris): 130 g a.i./ha
Executive summary:

A greenhouse trial was set up to obtain information on the sensitivity of 10 species of terrestrial plants. The effects on vegetative vigour were tested after application of the test substance at BBCH 12-14. The trial was conducted according to OPPTS 850.4150 and OECD guideline 227.


Soil (sieved to 2 mm) was natural soil from an agricultural area. The natural soil was silty loamy sand, which had been untreated with plant protection products and fertiliser for at least two years. For sugar beet cultivation the soil was pre-treated by steaming (>70°C) for more than 12 hours to reduce soil born diseases.


The trial was conducted in a greenhouse in Altfriesack, Brandenburg, Germany. This greenhouse is a chamber house with stony floor. Separate climate control for each chamber is based on an electronic system. All chambers can be illuminated and shaded when necessary.


The test was set up with 3 monocotyledonous and 7 dicotyledonous plant species. Germination tests were conducted prior to the start of the study to determine the viability of the seeds on filter paper.


A surplus of seeds was placed in each pot depending on germination ability. At BBCH 11-12 surplus plants were removed leaving the exact number of homogenous plants per pot for application.


Pots used were plastic containers with 10.5 cm, 12 cm or 14 cm in diameter (dependent on test species) not used before. Test crops were seeded several days before application to gain plants at BBCH stage 12-14.


The expected regime of temperatures between 12°C and 32°C was mainly held within this trial. The daily mean temperature ranged between 17.5°C and 25.2°C (extremes for short time only 11°C and 35°C. The daily mean relative humidity within the trial period ranged from 44.1 to 79.5% relative humidity (extremes 20 and 95%). The natural day length during the study period was adjusted to ≥16 hours by adding artificial light if outdoor light intensity was lower than 10 klux for 16 hours per day.


The test rates ranged between 32.5 g a.i./ha and 260 g a.i./ha. The factor between two subsequent rates was 2.


Plant survival was determined per pot 7, 14 and 21 days after treatment. For this the number of living and dead plants per pot was counted. To be considered alive, a plant had to have at least one green part on it.


Phytotoxicity assessments were done weekly after application following a scale of 0-10 where 0 means all plants dead and 10 excellent, good colour, no defects.


For biomass (fresh), all living plants of each pot were weighed together. Plants were cut directly at the soil surface. Dead plants were considered with a weight of 0 g. This assessment was done for all plant species within 24 hours after last irrigation. The weight was determined immediately after cutting the plant using a scale (type BA 310 P, Sartorius).


No plant mortality was observed for all tested plant species following the application of the test substance up to the highest tested rate at BBCH stage 12-14. The calculated NOER appeared to be higher than or equal 260 g a.i./ha. The ER50 value could not be calculated for plant survival, for any of the tested species.


The tested plant species showed no phytotoxic symptoms except soybean and sugar beet. Very slight chlorosis and deformation were observed in soybean in all treatments treated with the test substance. Sugar beet showed slight stunting after application of 260.0 g a.i./ha.


The plant biomass (shoot fresh weight) was determined 21 DAT. No influence of the test substance on plant weight was observed in oat, ryegrass, onion, oilseed rape, soybean, carrot, cucumber, sunflower and tomato. Sugar beet showed significant fresh weight reduction of around 14% after application of 260.0 g a.i./ha. The NOER for sugar beet was calculated to be 130.0 g a.i./ha. An ER50 was not detected for all tested plant species. No dose response could be found for all tested plant species.


Based on the results of this study, conducted under greenhouse conditions, it can be concluded that the test substance applied post emergence with rates up to 260 g a.i./ha did not cause adverse effects to plant mortality of all tested species and plant weight of oat, ryegrass, onion, oilseed rape, soybean, carrot, cucumber, sunflower and tomato.


The most sensitive plant species was found to be sugar beet. An ER50 level for fresh biomass reduction was not determined.