Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 270-336-2 | CAS number: 68425-16-1
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of polysulfide di-tert-nonyl to zebrafish (Danio rerio) was read across from polysulfide di-tert-dodecyl. Based on the loading rate, the results give a 96-hour LL50 of >100 mg/L. However, serious methodological deficiencies were found and the study is considered unreliable. A second study conducted with polysulfide di-tert-nonyl, showed the substance was not toxic at the limit of solubility, but concentrations in solution could not be determined as the limit of detection was above the water solubility limit. Chronic testing has been conducted with fish and is considered more appropriate for assessing this endpoint.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The toxicity of polysulfide di-tert-nonyl has been read across from polysulfide di-tert-dodecyl. Hoffmann (2010) is a GLP-compliant, static closed vessel limit test following OECD guideline 203. No further dose-response test was performed despite the limit test showing toxic effects, as the concentration tested was the threshold concentration and the highest concentration at which substances need to be tested.
There are considered to be significant methodological deficiencies with the study. Based on recent water solubility testing that has been conducted, the test item has a solubility of 0.26 µg/L. The method used for preparing the test solutions means that droplets may be present in the water column, and as no analytical monitoring was conducted the concentrations cannot be verified. The study is therefore considered to be unreliable, and chronic test results are used to assess toxicity to fish instead.
A second study is available, conducted with polysulfides, di-tert-nonyl. Thiebaud (2000) is a GLP-compliant study following OECD 203 guidelines. The concentration of the substance in the solutions could not be determined as the water solubility limit was below the limit of analytical detection. However, the substance was found not to be toxic at the limit of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
