Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-764-3 | CAS number: 2526-62-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-09-04 to 2009-09-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Known volume of test substance, Milli-RO water, synthetic sewage feed (16 ml) and activated sludge (200 ml) were mixed and made up to 500 ml with Milli-RO water in a 1 litre bottle. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: the sludge was coarsely sieved then washed
- Initial biomass concentration: 3.8 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Oxygen consumption was measured and recorded for approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
- Test temperature:
- 18.9-19.8°C
- pH:
- 8.2
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass, 300ml oxygen bottles, 1L test bottles
- Aeration: continuous during the 3 hour test period
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): two
- Biomass loading rate: 100 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ion-exchange cartridges (Milli-Q)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After the 3-hour contact time, a well mixed sample of the contents of the reaction mixture was poured into a 300 ml oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel. WTW inolab Oxi 730 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 100 mg/L only
- Justification for using less concentrations than requested by guideline: Performance of a limit concentration at 100 mg/l is acceptable, because 100 mg/l exceeds the expected concentration in a Sewage Treatment Plant. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The two controls had a difference in respiration rate of 0%, which is less than the 15% limit required by the guideline for a valid study.
- Results with reference substance (positive control):
- A positive control test with 3, 5-Dichlorophenol yielded an EC50 of 7.2 mg/L, which is within the accepted range of 5 to 30 mg/l required by the guideline for a valid study.
- Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiration inhibition 3hr EC50 value of >100 mg/l (loading rate) was determined in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. This indicates that the test substance was not toxic to waste water (activated sludge) bacteria at a loading rate of 100 mg/l.
Reference
Table 1: Results of the activated sludge respiration inhibition test
Test sample |
Loading rate (mg/l) |
Oxygen conc. at start (mg O2/l) |
Oxygen consumption (mg O2/l/h) |
% Inhibition respiration rate |
pH |
|
|
||||
Initial control |
0 |
7.2 |
34 |
- |
8.4 |
Final control |
0 |
7.0 |
34 |
- |
8.3 |
3-(triethoxysilyl)propiononitrile replicate (1) |
100 |
5.4 |
47 |
-38 (a) |
8.2 |
3-(triethoxysilyl)propiononitrile replicate (2) |
100 |
5.4 |
47 |
-38 (a) |
8.2 |
|
|
||||
3,5-dichlorophenol |
5 |
- |
- |
43 |
- |
12 |
- |
- |
61 |
- |
|
30 |
- |
- |
67 |
- |
Note: (a) A negative value indicates stimulation of the respiration rate of the sludge
Description of key information
Toxicity to microorganisms: 3 hour EC50 >100 mg/L (activated sludge respiration inhibition, OECD 209) for analogous substance 3-(triethoxysilyl)propiononitrile.
Key value for chemical safety assessment
Additional information
There are no microorganism toxicity data available for 3-(trimethoxysilyl)propiononitrile, therefore, good quality data for the analogous substance 3-(triethoxysilyl)propiononitrile (CAS 919-31-3) have been read across. Both substances share a common hydrolysis product, 3-(trihydroxysilyl)propiononitrile, and are also expected to hydrolyse to some extent within the timescale of the test. The other hydrolysis products are methanol and ethanol respectively.
Methanol and ethanol do not have adverse effects on microorganisms.
Both 3-(trimethoxysilyl)propiononitrile and 3-(triethoxysilyl)propiononitrile are within an analogue group of substances that exhibit no evidence of significant toxicity to microorganisms.
This wider analogue group for the toxicity to microorganisms endpoint consists of a number of sub-classes of substances. Read-across is carried out between substances with the same sub-class in most cases.
The table below presents microorganism toxicity data available for substances within the sub-class of chlorosilanes, siloxanes, silanes and alkoxysilanes etc, with nitrile functional group(s) in side chain after any very rapid hydrolysis has been accounted for.
3-(Trimethoxysilyl)propiononitrile and 3-(triethoxysilyl)propiononitrile are members of this sub-class.
It is therefore considered valid to read-across the results for 3-(triethoxysilyl)propiononitrile to fill the data gap for the registered substance. Additional information is given in a supporting report (PFA, 2013) attached in Section 13.
Table: Microorganism toxicity data available for organosilane substances with nitrile functional group
CAS |
Name |
Result: E(I)C50 (mg/L) |
Result: NOEC (or EC10/ EC20) (mg/L) |
Guideline Number |
Test method |
Species |
Duration |
Reliability |
919-31-3 |
3-(Triethoxysilyl)propiononitrile |
>100 |
|
OECD 209 |
ASRI |
|
3 hours |
1 |
An activated sludge respiration inhibition 3hr EC50 value of >100 mg/L (loading rate) was determined for 3-(triethoxysilyl)propiononitrile.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.