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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

in vitro DNA damage and/or repair study
Type of genotoxicity: DNA damage and/or repair
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: NTP-study, method and results are sufficiently described.

Data source

Reference Type:
Chromosome aberration and sister chromatid exchange test results with 42 chemicals
Anderson BE, Zeiger E, Shelby MD, Resnick MA, Gulati DK, Ivett JL, Loveday KS
Bibliographic source:
Environ. Mol. Mutagen. 16: 55-137

Materials and methods

Principles of method if other than guideline:
Sister chromatid exchange in CHO cells
GLP compliance:
not specified
Type of assay:
sister chromatid exchange assay in mammalian cells

Test material

Constituent 1
Reference substance name:
Methyl methacrylate
EC Number:
EC Name:
Methyl methacrylate
Cas Number:
methyl methacrylate


Species / strain
Species / strain / cell type:
other: CHO cells
Metabolic activation:
with and without
Metabolic activation system:
AROCLOR 1254 induced rat liver S9 mix
Test concentrations with justification for top dose:
1250 ug/ml - 5000 ug/ml

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
with and without
Cytotoxicity / choice of top concentrations:
Remarks on result:
other: all strains/cell types tested
Migrated from field 'Test system'.

Applicant's summary and conclusion

Interpretation of results (migrated information):
ambiguous clastogenic at high toxic doses

As clastogenic activity was found at probably high toxic doses, it is not possible to conlude that methyl methacrylate has a real direct clastogenic effect as it is probably secondary to cytotoxicity.
Executive summary:

In a cytogenetic test with CHO cells induction of chromosomal aberrations was bound to high doses which are assumed to be strongly cytotoxic. With S-9 mix treatment was for 2 h followed by 8 to 10 h recovery. Doses up to 1,600 μg/ml were negative, at 5000 μg/mL the frequency of aberrant cells was 30%; only one experiment was performed. Without S-9 mix, treatment time was 8 hours with 2.0 to 2.5 h recovery. Doses up to 500 μg/mL were negative, at 1600 and 3000 μg/mL aberration frequencies ranging from 5 to 10% were found. Data on cytotoxic effects were not given, however, it can be assumed from the data presentation and the general approach of the authors that the highest doses tested led to strong cytotoxic effects. Thus, methyl methacrylate showed clastogenic activity but at highly toxic doses therefore probably due to cytotoxicity more than a direct clastogenic effect.