Hydroxy(2-methylprop-2-enoato-O)zinc

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Similar to OECD-guideline 474, all relevant study details available but article in japanese.

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity of environmental substances

Author:

Hachiya N, Taketani A, Takizawa Y

Year:

1982

Bibliographic source:

Nippon Koshu Eisei Zasshi 29: 236-239

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

Principles of method if other than guideline:

Chromosome aberration rates and sister chromatid exchange frequency were examined in the peripheral lymphocytes of 38 male workers who were engaged in organic glass production and exposed to methyl methacrylate vapors.

GLP compliance:

not specified

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

Methyl methacrylate

EC Number:

201-297-1

EC Name:

Methyl methacrylate

Cas Number:

80-62-6

IUPAC Name:

methyl methacrylate

Details on test material:

no data

Test animals

Species:

mouse

Strain:

other: ddy

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

Olive oil

Duration of treatment / exposure:

4 doses

Frequency of treatment:

3 doses: once, 24 h before terminal sacrifice
1 dose: 4 split doses every 24 h, the last one 24 h before terminal sacrifice, total duration 5 d

Post exposure period:

24 h

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
Olive oil, 25 mL/kg
Basis:
actual ingested

Doses / concentrations 2

Remarks:

Doses / Concentrations:
1130, 2260, 4520 mg/kg bw
Basis:
actual ingested

Doses / concentrations 3

Remarks:

Doses / Concentrations:
4 x 1130 mg/kgbw
Basis:
actual ingested

No. of animals per sex per dose:

6 (repeated treatment of 4 x 1130 mg/kgbw : 5)

Control animals:

yes

Positive control(s):

3 mg Mitomycin C, single dose by i.p. administration 24 h prior to preparation

Examinations

Tissues and cell types examined:

Sampling time for bone marrow: 3 single doses - 24 h post-administration; for repeated administration: 5 days after first administration.

Statistics:

according to Kastenbaum/Bowman

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

no effects

Vehicle controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

The substance has been administered by gavage as a solution in olive oil in 3 single doses ranging from 1130 mg/kg to 4520 mg/kg (0.5 LD50) 24 h prior to preparation of the bone marrow. A separate group of 5 animals was administered 4 doses of 1130 mg/kg 96, 72, 48 and 24 h prior to preparation. Olive oil (25 ml/kg) was used as the solvent control and mitomycin C (3 mg/kg, i.p.) as the positive control. 2000 erythrocytes were evaluated per animal (12000/10000 per dose). No increase in micronucleated polychromatic erythrocytes was observed at any dose, while an induction of micronuclei was seen in the positive control. MMA was not mutagenic in vivo under test conditions.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
Methy lemthacrylate was not mutagenic in vivo under test conditions.

Executive summary:

The substance has been administered by gavage as a solution in olive oil in 3 single doses ranging from 1130 mg/kg to 4520 mg/kg (0.5 LD50) 24 h prior to preparation of the bone marrow. A separate group of 5 animals was administered 4 doses of 1130 mg/kg 96, 72, 48 and 24 h prior to preparation. Olive oil (25 ml/kg) was used as the solvent control and mitomycin C (3 mg/kg, i.p.) as the positive control. 2000 erythrocytes were evaluated per animal (12000/10000 per dose). No increase in micronucleated polychromatic erythrocytes was observed at any dose, while an induction of micronuclei was seen in the positive control. The substance has been administered by gavage as a solution in olive oil in 3 single doses ranging from 1130 mg/kg to 4520 mg/kg (0.5 LD50) 24 h prior to preparation of the bone marrow. A separate group of 5 animals was administered 4 doses of 1130 mg/kg 96, 72, 48 and 24 h prior to preparation. Olive oil (25 ml/kg) was used as the solvent control and mitomycin C (3 mg/kg, i.p.) as the positive control. 2000 erythrocytes were evaluated per animal (12000/10000 per dose). No increase in micronucleated polychromatic erythrocytes was observed at any dose, while an induction of micronuclei was seen in the positive control. Methyl methacrylate was not mutagenic in vivo under test conditions.