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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
Analytical monitoring:
Details on sampling:
- Concentrations: All test concentrations were analysed.
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution I was prepared by dissolving 20 mg test substance in tetrahydrofuran to a final volume of 50 mL (conc. 400 mg/L). Stock solution II was prepared separately for each test concentration by mixing different volumes of stock solution I with tetrahydrofuran. To prepare the test solutions, the stock solutions were diluted by mixing 500 microL stock solution II with 5000 mL dechlorinated water
- Controls: The test solution of Control was only dechlorinated water. For the solvent control 500 microL tetrahydrofuran was mixed with 5000 mL dechlorinated water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tetrahydrofuran
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 microL/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): colorles, no suspended solids, floating solids or precipitations observed
Test organisms (species):
Oryzias latipes
Details on test organisms:
- Common name: Medaka
- Source: Tsunashima Fishing Co., Yokohama, Kanagawa, Japan
- Age at study initiation (mean and range, SD): a year or less after hatching
- Length at study initiation (length definition, mean, range and SD): 2.20 - 2.64 cm
- Weight at study initiation (mean and range, SD): 0.092 - 0.165 g
- Feeding during test: none

- Acclimation period: 13 Feb - 20 Feb 2012
- Acclimation conditions (same as test or not): no, flow-through
- Type and amount of food: TetraMin, TetraWerke, 2% of the body weight/day (approx.), until 24 h before exposure
- Health during acclimation (any mortality observed): mortality less than 5%

Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
51 mg CaCO3/L
Test temperature:
23.0 - 24.0 °C
7.2 - 7.7
Dissolved oxygen:
6.1 - 8.3 mg/L
Nominal and measured concentrations:
Nominal: 0.00080, 0.0014, 0.0025, 0.0045 and 0.0080 mg/L (limit of water solubility)
Measured: 0.000362, 0.000652, 0.00114, 0.00216 and 0.00350 mg/L
Details on test conditions:
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

- Source/preparation of dilution water: Yokohama city tap water treated with an activated charcoal filter and sodium thiosulfate
- Total organic carbon: 0.3 mg/L
- Metals: below detection limit
- Chlorine: < 0.1 mg/L
- Alkalinity: 38 mg CaCO3/L
- Calcium: 15 mg/L
- Magnesium: 3.4 mg/L
- Conductivity: 14 mS/m
- Intervals of water quality measurement: Temperature, dissolved oxygen and pH were measured daily.

- Adjustment of pH: no
- Photoperiod: 16 h light 8 h dark
- Light intensity: 1000 lux or less, fluorescent light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Test fish were observed for swimming behaviour, appearance and respiration at 3, 24, 48, 72 and 96 hours after the start of exposure.

- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 0.00080 and 0.0080 mg/L
- Results used to determine the conditions for the definitive study: no mortality occurred
96 h
Dose descriptor:
Effect conc.:
> 0.004 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Highest attainable concentration in test water
Details on results:
- No dead fish and no abnormal symptoms in living fish were observed in the control, solvent control or the test concentrations.

Description of key information

Up to the highest attainable concentration no LC50 could be reached.

96h-LC50 > 0.00371 mg/L (OECD 203; Oryzias latipes)

Key value for chemical safety assessment

Additional information

One study is available on the short-term toxicity of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl 2-chloropropenoic acid ester (CAS 96383-55-0) to fish. The study was conducted according to OECD guideline 203 and GLP (Niikura, 2012). The test organism Oryzias latipes was exposed to the test substance under semi-static conditions at test concentrations of 0.000362, 0.000652, 0.00114, 0.00216 and 0.00350 mg/L (mean measured) for 96 hours. As the test substance is poorly soluble in water, tetrahydrofuran was used as a solvent for the preparation of the test solutions. At the end of the exposure period no mortality or abnormal behavior were observed up to the highest attainable test concentration. Therefore, the LC50 (96h) is determined to be above the highest attainable test concentration, corresponding to a measured value of > 0.0035 mg/L.