Trisodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonatonaphthyl]azo]-2,5-dimethylphenyl]azo]benzene-1,4-disulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD5

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Method A-15

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name : FAT 40075/A
Purity: 74.5 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

5 d

Initial conc.:

50 mg/L

Parameter followed for biodegradation estimation:

O2 consumption

Remarks on result:

not measured/tested

Key result

Parameter:

BOD5

Value:

56 mg O2/g test mat.

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 of FAT 40075/A is found to be 56 mg O2/g.

Executive summary:

BOD5 of FAT 40075/A was determined according to in house Ciba Geigy Method A.15, which is comparable to internationally accepted guidelines. Based on these investigations, the BOD5 of test substance was found to be 56 mg O2/g. Considering the COD value determined with the test substance which is 898 mg O2/g, the resulting BOD5/COD quotient is 0.06, indicating that the test substance is not readily biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Method A-16

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 20033/A
Purity: 74.5 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Parameter followed for biodegradation estimation:

O2 consumption

Remarks on result:

not measured/tested

Parameter:

COD

Value:

898 mg O2/g test mat.

Interpretation of results:

not readily biodegradable

Conclusions:

COD of FAT 40075/A is found to be 898 mg O2/g.

Executive summary:

COD of FAT 40075/A was determined according to CIBA -GEIGY internal method A.16. Based on the findings of the study, the COD of test substance was found to be 898 mg O2/g. Considering the BOD5 value determined with the test substance (56 mg/ gO2), the resulting BOD5/COD quotient is 0.06, indicating that the test substance is unlikely to be biodegradable.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Remarks:

TOC removal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Method A-12

Version / remarks:

Comparable to internationally accepted guidelines.

Principles of method if other than guideline:

Analysis of the test substance entering and leaving the laboratory wastewater treatment unit on the basis of total organic carbon (TOC).

GLP compliance:

no

Specific details on test material used for the study:

Name : FAT 40075/A
Purity: 74.5 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

No data

Duration of test (contact time):

31 d

Initial conc.:

64.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

TOC removal

Parameter:

% degradation (TOC removal)

Value:

14

Sampling time:

31 d

Validity criteria fulfilled:

no

Interpretation of results:

not inherently biodegradable

Conclusions:

Under the test conditions, the substance was not inherently biodegradable.

Executive summary:

The TOC removal of FAT 40075/A was determined according to CIBA internal Method A-12. The TOC removal of FAT 40075/A was found to be 14 % after 31 days. Under the test conditions, the substance was found to be not inherently biodegradable.

Description of key information

FAT 40075 is considered as not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

A study was conducted to evaluate the biodegradation potential of the test substance (of 74.5 % purity) via BOD5 and COD determination. The BOD5 was determined to 56 mg O2/g test material, the COD was calculated to 878 mg O2/g test material. The ratio of these values was equivalent to 0.06 (= 6 %), indicating that the substance was not readily biodegradable.