Pyruvaldehyde

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-09-03 to 1987-10-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP but internally audited. Scientifically acceptable study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

not applicable

Principles of method if other than guideline:

3 rats / sex instead of 5 rats / sex

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pyruvaldehyde

EC Number:

201-164-8

EC Name:

Pyruvaldehyde

Cas Number:

78-98-8

Molecular formula:

C3H4O2

IUPAC Name:

2-oxopropanal

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, 7950 Biberach, Germany
- Age at study initiation: 8 to 9 weeks
- Mean weight at study initiation: males: 270g, females: 196 g (no animal exceeding +/- 20% of the mean weight)
- Fasting period before study: 16 h
- Housing: Stainless steel wire mesh cage (Type DK III)
- Diet: ad libitum (except during exposition)
- Water: ad libitum (except during exposition)
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature: 20 - 24°C
- Humidity: 30 - 70 %
- Air changes: not specified ("fully air conditioned")
- Photoperiod: 12 hrs dark / 12 hrs light

IN-LIFE DATES: From: To: 1987-09-03 to 1987-09-17

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: glass frit (grain size 90-150 µm.) filled with product, dissipated into glass tubes containing animals; exhaust vent
- Exposure chamber volume: not specified
- Method of holding animals in test chamber: not restrained
- Source and rate of air: 200 L / h

- System of generating aerosols: air flowing through product in glass frit
- Treatment of exhaust air: vented
- Temperature, humidity, pressure in air chamber: ambient (laboratory conditions: 19 to 25°C)

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

7 h

Concentrations:

mean: 13,6 mg/L (calculated by a net total of 19.1 g test item spent in 1400 L air)

No. of animals per sex per dose:

3 males and 3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: clinical signs on workdays, fatalities daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 13.6 mg/L air

Based on:

test mat.

Remarks on result:

other: nominal concentr.

Mortality:

no

Clinical signs:

other: During exposure: eyelid closure, respiratory rate increased, panting, respiratory sounds, reddish discharge from nose After exposure: respiratory sounds, reddish discharge from nose (tested positive for blood), stilted gait

Body weight:

not documented

Gross pathology:

no abnormalities detected

Other findings:

no