2-(2-butoxyethoxy)ethyl 6-propylpiperonyl ether

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EPA OPP 72-1 (Fish Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Cyprinodon variegatus

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21.0 – 22.5 °C

pH:

8.1 – 8.3

Dissolved oxygen:

5.8 – 7.6 mg/L

Details on test conditions:

Initial concentrations of test substance 1.3, 2.2, 3.6, 6.0 and 10.0 ppm of PBO

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2.97 mg/L

Remarks on result:

other: (measured/nominal)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.94 mg/L

Remarks on result:

other: (calculated)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

>= 5.24 mg/L

Remarks on result:

other: (measured/nominal)

Sublethal observations / clinical signs:

For the treated groups with nominal PBO concentrations of 1.3, 2.2, 3.6, 6.0 and 10.0 ppm the mean measured concentrations of radioactivity were 0.92, 1.84, 2.97, 5.24 and 6.62 ppm, respectively. In the solvent control group all animals appeared healthy and normal throughout the test. Signs of abnormal behaviour among fish exposed to PBO at 1.84 ppm were observed by 72 hours. At concentrations greater than or equal to 2.97 ppm effects were noted within approximately two hours of test initiation. By the end of the test all of the fish at concentrations greater than 2.97 ppm were dead or exhibited signs of abnormal behaviour.No fish died at PBO concentration levels of 0.92, 1.84 and 2.97 ppm. At the PBO concentration level of 5.24 ppm the percent mortalities by 48, 72 and 96 hours of exposure were 20, 80 and 100 %. At the highest exposure level of 6.62 ppm the percent mortalities were 25, 90 and 100 % after 24, 48 and 72 hours of exposure.

Validity criteria fulfilled:

yes

Conclusions:

The results of this study demonstrated that the 96 h no mortality concentration of PBO for sheepshead minnows was 2.97 ppm. The 96 h LC50 of PBO was 3.94 ppm (3.94 mg/L).

Description of key information

For sheepshead minnows the 96 h LC50 of PBO was 3.94 ppm (3.94 mg/L).

For bluegill the 96 h LC50 of PBO was 5.37 ppm (5.37 mg/L).

For Rainbow Trout (Oncorhynchus mykiss) the LC50 96h was 6.12 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.94 mg/L