1-bromopentane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-bromopentane

EC Number:

203-776-0

EC Name:

1-bromopentane

Cas Number:

110-53-2

Molecular formula:

C5H11Br

IUPAC Name:

1-bromopentane

Details on test material:

Appearence: colorless liquid
Purity: > 98%
Lot number: Lab 08 02

Test solutions

Details on test solutions:

Ultrapur water.
Water was ultrafiltered, passed on active carbon and ion exchange resin then filtered by a FG Millipore membrane (0.22 µm).

A saturated solution was prepared by puting 100 g of Bromo-1-pentane in 1 liter of solution under agitation during 18 hours with a temperature of 20°C.
The solutions with the concentration range were prepared by diluting the saturated solution with ultrapur water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Strauss cultived by the laboratory.
Organisms of less than 24 hours were selected by screening through a 0.56 mm bolt cloth.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

Not done

Test conditions

Hardness:

No data

Test temperature:

Between 19 and 21°C

pH:

pH variation between 7.50 and 7.91

Dissolved oxygen:

Concentrations between 8.0 and 8.7 mg/l

Salinity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

4 flasks of 5 daphnia were prepared for each concentration and the control test.

Reference substance (positive control):

yes

Remarks:

Dichromate de potassium: EC50- 24 h = 0.94 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

4.5 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not determined

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Not determined

Results with reference substance (positive control):

Immobilisation after 48 hours at 5%

Reported statistics and error estimates:

95% CL not detrmined because there are not enough points.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Bromo-1-pentane have a CE50 24h of 4.5 mg/l and a CE50 48h of 1.9 mg/l. It has been to classify as Dangerous for environment Tox 2.

Executive summary:

The study was conducted under EU Method C2

After 48 hours exposure to the test substance, the CE 50 was determined to be 1.9 mg/l.