Naphthalenesulfonic acid, di-C9-rich C8-10-branched alkyl derivs.

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study follows generally accepted practices. Result (>2 ml/kg) gives clear indication of low toxicity by dermal route.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: This test methodology follows the procedure of the US Federal Hazardous Substances Act (1973) to determine substance toxicity.

GLP compliance:

no

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid

EC Number:

939-714-0

Cas Number:

1474044-77-3

Molecular formula:

C18H30SO3 to C40H67SO3

IUPAC Name:

di C8-C10, branched, C9 rich, alkylnaphthalene sulphonic acid

Test material form:

other: applied as 50% solution in light paraffinic petroleum oil

Details on test material:

50% DNNSA (DI C8-C10, BRANCHED, C9 RICH, ALKYLNAPHTHALENE SULFONIC ACID)
50% light paraffinic petroleum oil

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

not specified

Details on test animals or test system and environmental conditions:

Ten healthy mature albino rabbits of New Zealand variety were selected from the stock colony for this study. They were housed in individual
stainless steel cages. Purina Rabbit Chow and water were available ad libitum.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The compound was applied to the moistened clipped skin of ten rabbits (five intact and
five abraded). The rabbits then were wrapped with a rubber dam. After 24 hours, the rubber dam was removed and the residue gently washed off.

Duration of exposure:

24 hour exposure

Doses:

2 ml / kg

No. of animals per sex per dose:

10 (5 abraded and 5 unabraded)

Control animals:

no

Details on study design:

The compound was applied at a dose of 2 ml per kg body weight to the moistened clipped skin of ten rabbits (five intact and
five abraded). The rabbits then were wrapped with a rubber dam. After 24 hours, the rubber dam was removed and the residue gently washed off.
The test site was examined for erythema and edema and scores were recorded daily for fourteen days. Body weights were recorded on Days 0 and 14. Rabbits were observed daily for signs of toxicity. All surviving animals were sacrificed at 14 days after treatment and a thorough necropsy was conducted.

Statistics:

Average body weights computed.

Results and discussion

Preliminary study:

Dermal LD50 > 2 ml/kg
Dermal LD0 > 2 ml/kg

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

LD0

Effect level:

> 2 mL/kg bw

Based on:

test mat.

Effect levels 2

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 2 mL/kg bw

Based on:

test mat.

Mortality:

None.

Clinical signs:

other: None.

Gross pathology:

No abnormal effects observed.

Any other information on results incl. tables

Body Weights (g)

Animal	Skin	Day 0	Day 14
1	Abraded	2175	2230
2	Abraded	2370	2410
3	Abraded	2940	2990
4	Abraded	1915	2100
5	Abraded	2810	2885
6	Intact	2425	2510
7	Intact	2270	2340
8	Intact	2390	2465
9	Intact	2800	2895
10	Intact	2630	2720
Ave.		2472.5	2554.5

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Criteria used for interpretation of results: other: Guidelines established by the US Federal Hazardous Substances Act (1973)

Conclusions:

The dermal LD0 and LD50 are greater than 2 ml/kg.

Executive summary:

Ten healthy mature albino rabbits of New Zealand variety were selected from the stock colony for this study. They were housed in individual stainless steel cages. Purina Rabbit Chow and water were available ad libitum. The compound was applied at a dose of 2 ml/kg (liquid) to the moistened clipped skin of ten rabbits (five intact and five abraded). The rabbits then were wrapped with a rubber dam. After 24 hours, the rubber dam was removed and the residue gently washed off. The test site was examined for erythema and edema and scores were recorded daily for fourteen days. Body weights were recorded on Days 0 and 14. Rabbits were observed daily for signs of toxicity. All surviving animals were sacrificed at 14 days after treatment and a thorough necropsy was conducted.

The rabbits showed no signs of toxicity and no abnormal findings were noted in the necropsy. The dermal LD0 and LD50 are greater than 2 ml/kg.