Butanedioic acid, 2-sulfo-, 1(or 4)-[2-(C12-18 and C18-unsatd. amido)ethyl] esters, disodium salts

Short-term toxicity to fish

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

32 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Butanedioic acid, 2(or 3)-sulfo-, 4-[2-[(1-oxo(C12-C18(even numbered) and C18unsaturated)alkyl))amino]ethyl]esters, disodium salts to zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The following nominal concentrations of Butanedioic acid, 2(or 3)-sulfo-, 4-[2-[(1-oxo(C12-C18(even numbered) and C18unsaturated)alkyl))amino]ethyl]esters, disodium salts were tested: 0.76, 2.4, 7.6, 24 and 76 mg test item/L corresponding to 0.32, 1.0, 3.2, 10 and 32 mg solid content/L. Additionally, a control (test water without test item) was tested in parallel. The measured concentrations of the 32 mg solid content/L was within 94 and 101 % of the nominal concentrations. Consequently, the biological results are related to the nominal test item concentrations.

In the control and up to and including the highest test concentration 32 mg solid content/L, all fish survived until the end of the test and no visible abnormalities were observed. The 96 hour NOEC and LC₀of Butanedioic acid, 2(or 3)-sulfo-, 4-[2-[(1-oxo(C12-C18(even numbered) and C18unsaturated)alkyl))amino]ethyl]esters, disodium salts to zebra fish were both determined to be 32 mg solid content/L. The 96 hour LC₅₀was determined to be > 32 mg solid content/L.

The results of this study are considered relevant and reliable for the risk assessment.