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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Adequacy of study:
other information

Data source

Reference
Title:
No information
Author:
Ministry of Environment, Japan(1997a): unpublished report on|Fish, Acute Toxicity Test (No. 6B715G)

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(dimethylamino)ethyl acrylate
EC Number:
219-460-0
EC Name:
2-(dimethylamino)ethyl acrylate
Cas Number:
2439-35-2
Molecular formula:
C7H13NO2
IUPAC Name:
2-(dimethylamino)ethyl acrylate
Details on test material:
IUCLID4 Test substance: as prescribed by 1.1 - 1.4

TS-Freetext:
SOURCE: Wako Pure Chemical Industries, LTD (Japan)
PURITY: 99.9%
IMPURITY/ADDITIVE/ETC.: not described
ANY OTHER INFORMATION: Lot No.SKK4558, got on Dec.25,1996

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Oryzias latipes

Study design

Test type:
semi-static

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8.49 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
RESULTS: EXPOSED

- Measured concentrations: in these stock solution 
  (10,000 mg/L)
  immediately after the preparations 

   Nominal         Measured concentration [mg/L]
   concentration   1st preparation (% of nominal) 
   [mg/L]
    10,000                      8,930
                                (89) 
                                            
 ND = 10 mg/L

- Effect data (Mortality): 
     
   Nominal       Cumulative Mortality (% Mortality)
   concentration       
   [mg/L]        24 hr    48 hr      72 hr    96 hr
   Control       0  (0)    0  (0)    0  (0)    0  (0) 
    6.00         0  (0)    0  (0)    0  (0)    0  (0) 
   12.0          4  (40)   0  (100) 10  (100) 10  (100)
   24.0         10  (100) 10  (100) 10  (100) 10  (100)

 24 hr LC50 = 13.1 mg/L (95%c.l. = 6.00 - 24.0 mg/l) 
 48 hr LC50 = 8.49 mg/L (95%c.l. = 6.00 - 12.0 mg/L)
 72 hr LC50 = 8.49 mg/L (95%c.l. = 6.00 - 12.0 mg/L)
 96 hr LC50 = 8.49 mg/L (95%c.l. = 6.00 - 12.0 mg/L)
 96 hr lowest concentration resulting in 100% mortality 
 = 12.0 mg/L
 96 hr highest concentration resulting in 0% mortality 
 =6.0 mg/L

- Other effects: 
   Nominal        Symptom of toxicity observed
   concentration       
   [mg/L]        24 hr  48 hr  72 hr  96 hr
   Control          N     N      N      N
   Solvent control  N     N      N      N
    6.00            N     N      N      N
   12.0            AS-1   --     --     -- 
                   AQ-1
   24.0            --     --     --     --
   48.0            --     --     --     --
   96.0            --     --     --     --

Note; N : No toxicological symtom was observed
     -- : All fish were dead at this observation time  
     AS : abnormal swimming
     AQ : paralyzation


RESULTS: CONTROL 
- Nature of adverse effects: none 


RESULTS: TEST WITH REFERENCE SUBSTANCE
- Reference substance: CuSO4·5H2O
- Results: 96 hr LC50 = 0.44 mg/L and 0.80 mg/L

Applicant's summary and conclusion