Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Adequacy of study:
other information

Data source

Reference
Title:
No information
Author:
Ministry of Environment, Japan(1997e): unpublished report on|Daphnia magna Reproduction Test (No.6B702G)

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Version / remarks:
Cited as OECD Guide-line 202, part 2 (Daphnia sp., Reproduction Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(dimethylamino)ethyl acrylate
EC Number:
219-460-0
EC Name:
2-(dimethylamino)ethyl acrylate
Cas Number:
2439-35-2
Molecular formula:
C7H13NO2
IUPAC Name:
2-(dimethylamino)ethyl acrylate
Details on test material:
TS-Freetext:
SOURCE: Wako Pure Chemical Industries, LTD (Japan)
PURITY: 99.9%
IMPURITY/ADDITIVE/ETC.: not described
ANY OTHER INFORMATION: Lot No.SKK4558, got on Dec.25,1996

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
10 mg/L
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
6.27 mg/L
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
3.94 mg/L

Any other information on results incl. tables

RS-Freetext:
RESULTS: RANGE FINDING TEST: 48hr EiC50 = 9.92 mg/L
RESULTS: EXPOSED
- Nominal/measured concentrations: The concentration was
measured immediately after the preparation.

  Nominal              Measured concentration [mg/L] (%)
  concentration       Preparation
  [mg/L]              1st                  2nd
-------------------   ---------------      --------------
  1000                826 (83%)            832 (83%)
----------------------------------------------------------
- Effect data: 
    21 day LC50  = 3.94 mg/L ( 95% c.l.: 3.26 - 4.84 mg/L) 
    21 day ErC50 = 6.27 mg/L ( 95% c.l.: 0.100 - 10.0 mg/L) 
    21 day NOECr = 3.00 mg/L
    21 day LOECr = 10.0 mg/L
- cumulative reproduction: 
  (1) Cumulative number of dead parental Daphnia and
mortality during
     exposure of 21day

     Nominal              Number of               Mortality 
     concentration   dead parental    (%)   
     [mg/L]             Daphnia
------------------   -------------    ---------------------
control              1                (  3)  at 20th day
0.100                     1                      (  3)  at 20th day 
0.300                     2                      (  5)  at 21th day
1.00                     4                      ( 10)  at 21th day
3.00                     6                      ( 15)  at 20th day
10.0                     40                      (100)  at 18th day
----------------------------------------------------------
  (2) mean days required to first brood production during 
     exposure to 2-(dimethylamino)ethyl acrylate:
                        
      Nominal               Mean days
      concentration
      [mg/L]        
      ------------    ----------------
      control         7.25
      0.100           7.00
      0.300           7.00
      1.00            7.00
      3.00            7.00
      10.0            7.50
        ------------------------------------
(3) mean cumulative number of juveniles produced per adult
      during exposure after 21 day

        Nominal         Number
        concentration
        [mg/L]        
        -------------   -----------
        control         138.1
        0.100           131.8
        0.300           80.2                                
       1.00            134.0
        3.00            149.4
        10.0            13.1
        --------------------------------
RESULTS: TEST WITH REFERENCE SUBSTANCE
- Results: K2Cr2O7 pure grade: 48 hr EiC50 = 0.23 mg/L
(immobility data)
STATISTICAL RESULTS:

Applicant's summary and conclusion