Reaction mass of disodium sulphide and sodium carbonate and sodium hydrogensulphide

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Short description of an in-vivo study performed similar to the OECD guideline. However several information details are missing in this summary.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

yes

Remarks:

as only a summary report is available several information details were not given, e.g. controls, detailed application description, information on washing, individual results.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium hydrogensulphide

EC Number:

240-778-0

EC Name:

Sodium hydrogensulphide

Cas Number:

16721-80-5

Molecular formula:

NaHS

IUPAC Name:

sodium hydrogensulfide

Constituent 2

Reference substance name:

sodium sulfhydrate

IUPAC Name:

sodium sulfhydrate

Details on test material:

- Name of test material (as cited in study report): Sulfhydrat F150, Natriumsulfhydratlauge
- Molecular formula (if other than submission substance): NaHS (aqueous solution)
- Molecular weight (if other than submission substance): 56.06 g/mol
- Substance type: technical product
- Physical state: yellow liquid
- Storage condition of test material: In tightly closed original container, in a cool, well-ventilated place; protect from sunlight; do not store with acids; do not store together with oxidizing agents
- Other: pH value: 12 - 13

Test animals / tissue source

Species:

rabbit

Strain:

Himalayan

Details on test animals or tissues and environmental conditions:

TEST ANIMALS-albino
- Housing: in single cages
- Diet: mixed diet ERKA 8300 (Futtermittelwerke Robert Koch oHG/Hamm, Germany), ad libitum
- Water: tap water, ad libitum

Test system

Vehicle:

physiological saline

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted Sulfhydrat solution (ca. 31-32% NaHS), 10 % Sulfhydrat solution, 5 % Sulfhydrat solution, and 1 % Sulfhydrat solution

Duration of treatment / exposure:

single treatment

Observation period (in vivo):

144 hours

Number of animals or in vitro replicates:

6

Details on study design:

- the test substance was placed in the conjunctival sac
- eyes were examined at 1, 3, 7, 24, 48, 72 and 144 hours after test substance application
-for the 24 hours examination, the eyes were further examined with the aid of fluorescein

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

other: no data

Remarks on result:

other: After 6 days the observation was stopped. The effect of the undiluted Sulfhydrat solution (31-32 % NaHS) was not reversible at this time. Even if the observation period should be up to 21 days this result is interpreted as a corrosive effect.

Results 2

Remarks on result:

other: Short description of an in-vivo study performed similar to the OECD guideline. However several information details are missing in this summary.

Irritant / corrosive response data:

- the undiluted sample resulted in general to deep red colouring and an obvious lacteal swelling of the conjunctivae for 144 hours; furthermore a mild to severe opaque cornea was observed
- the 10 % solution resulted in a mild general red colouring of the conjunctivae for a little bit longer than 24 hours; in addition two rabbits showed a very mild redness of the conjuntivae for about three hours
- the 5 % solution resulted in a very mild redness of conjunctivae in 3 rabbits for approximately 3 hours
- the 1 % solution resulted in no irritating or corrosive effects

Any other information on results incl. tables

The authors considered the undiluted product as strongly irritant and the 5% and 10 % solutions as very slight to mildly irritant to eyes.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye)

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

After 6 days the observation was stopped. The effect of the undiluted Sulfhydrat solution (31-32 % NaHS) was not reversible at this time. Even if the observation period should be up to 21 days this result is interpreted as a corrosive effect. The 1 % solution can be judged as not corrosive and not irritant.