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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

Based on the results obtained in studies conducted according to OECD Guideline 202 (Biofert Plusz) and a national guideline (L-threonine mother liquor and modified waste), respectively, the 48 h EC50=677 mg dry matter/L (measured concentration) found for Biofert Plusz with Daphnia magna as test organism can be regarded as worst case for the toxicity of L-threonine mother liquor.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
677 mg/L

Additional information

The acute toxicity of L-threonine mother liquor and modified waste (used microbiological material) to Daphnia magna was investigated in studies conducted according to a national guideline. The 48 h EC50 values were determined to be comparable (L-threonine mother liquor: 48 h EC50=14 mL/L; modified waste: 48 h EC50=11.61 mL/L nominal each).

The acute toxicity of Biofert Plusz (mixture of L-threonine mother liquor and L-threonine biomass; L-threonine biomass is also named as modified waste (used microbial material sterilized), mortified biomass, mortified biomass of E. coli (K12), biomass concentrate of threonine production stem) towards Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). 20 daphnids (5 daphnids/replicate) were exposed to Biofert Plusz for 48 h at nominal concentrations ranging from 469 to 3559 mg/L under semi-static conditions. The test material concentration was determined by HPLC based on L-threonine (main component) measurements. The 48 h EC50 was determined to be 2410 mg fresh material/L, corresponding to 677 mg dry matter/L (nominal). Moreover, a 48 h NOEC=444 mg dry matter/L and a LOEC=667 mg dry matter/L were found.

In conclusion of the available data, it can be assumed that the result obtained with Biofert Plusz represents a worst case situation for the test material under investigation (L-threonine mother liquor).