Thiodiethylene bis[3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1984-06-25 to 1984-07-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study with analytics. Use of vehicle allowed testing of high concentrations well above the limit of water solubility (water solubility = 5-7 µg/L). Vehicle control showed no negative effects.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 12 May 1981

Deviations:

yes

Remarks:

: vehicle concentration > 100 mg/L (1900 mg/L)

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L (nominal); 57 mg/L (actual)
- Sampling time: at 0 h and 96 h exposure

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 1900 mg DMF; 0.4 mg Marlopon AT50 per L water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none; the test substance appeared homogenously distributed at the test concentration
- Controls: blank and vehicle control

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West-Aquarium / D-3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 25 - 33 mm (average: 28 mm)
- Weight at study initiation (mean and range, SD): 0.2 g (0.2 - 0.3 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 159 d
- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

152 mg CaCO3/L

Test temperature:

23 ± 1 °C

pH:

8.0

Dissolved oxygen:

80 - 99 %

Nominal and measured concentrations:

nominal: 100 mg/L
actual (geometric mean of measured concentrations at 0 h and 96 h): 57 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 L filled with 15 L (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.2 g/L

TEST MEDIUM / WATER PARAMETERS
- Watear: dechlorianted tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, O2, temperature - daily

OTHER TEST CONDITIONS
- Light: Fluorescent light, 16 h daily

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 57 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 57 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 57 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Mortalities vehicle: 10%
- Other adverse effects control: none
- Effect concentrations exceeding solubility of substance in test medium: yes

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1984-06-25 to 1984-07-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study with analytics. Use of vehicle allowed testing of high concentrations well above the limit of water solubility (water solubility = 5-7 µg/L). Vehicle control showed no negative effects.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 12 May 1981

Deviations:

yes

Remarks:

: vehicle concentration > 100 mg/L (1900 mg/L )

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentration of vehicle in test medium: 1900 mg DMF; 0.4 mg Marlopon AT50 per L water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler / CH-4314 Zeiningen
- Length at study initiation (length definition, mean, range and SD): 49 mm (47 - 50 mm)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 27 d
- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

152 mg CaCO3/L

Test temperature:

15 ± 1 °C

pH:

7.9-8.0

Dissolved oxygen:

73 - 98 % (during test)

Nominal and measured concentrations:

nominal: 100 mg/L
actual (geometric mean): 61 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 L filled with 15 L (36/22/25 cm)
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Watear: dechlorianted tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, O2, temperature - daily

OTHER TEST CONDITIONS
- Light: Fluorescent light, 16 h daily

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 61 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 61 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 61 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Effect concentrations exceeding solubility of substance in test medium: yes

Description of key information

With high probability acutely not harmful to fish. 

Additional information

In a 96-h acute toxicity study, zebra-fish (Brachydanio rerio) were exposed to test item at nominal concentrations of 0 (control, solvent control) and 100 mg/L (nominal) under static conditions. The 96-h LC50 was > 57 mg/L (average of measured concentration). A supporting study on rainbow trout according to OECD 203 did not reveal any toxic effects.

Neither of the studies revealed toxic effects in the range of water solubility.