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Short-term toxicity to fish

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Description of key information

LC50: 57 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
57 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Reaction products of ricinoleic acid with 2-aminoethanol and maleic acid and sodium hydrogensulfite zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992). The following nominal concentrations of Reaction products of ricinoleic acid with 2-aminoethanol and maleic acid and sodium hydrogensulfite were tested: 2.5, 8.0, 25, 80 and 249 mg test item/L (corresponding to 1.0, 3.2, 10, 32 and 100 mg solid content/L). Additionally, a control (test water without test item) was tested in parallel. In the analyzed biologically relevant test media samples (32 and 100 mg solid content/L) from the start and end of the first and last test medium renewal periods the measured test item concentrations ranged between 83 and 103% of the nominal values. Consequently, the test water renewal ensured constant concentrations of the test item during the test and the reported biological results are related to the nominal test item concentrations. In the control and at the test concentrations up to and including 80 mg test item/L (32 mg solid content/L), all fish survived until the end of the test and no visible abnormalities were observed in the test fish. At the highest test concentration of 249 mg test item/L (100 mg solid content/L), all test fish showed one visible abnormality (apathy). After 96 hours test duration, all seven test fish died at this test concentration.

The 96‑hour LC50of Reaction products of ricinoleic acid with 2-aminoethanol and maleic acid and sodium hydrogensulfite was calculated to be 57 mg solid content/L with a 95% confidence interval of 32 - 100 mg solid content/L.

The results of this study are considered relevant and reliable for the risk assessment.