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Toxicity to aquatic algae and cyanobacteria

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Description of key information

ErC10: 6 mg/L
ErC50: 87 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
87 mg/L
EC10 or NOEC for freshwater algae:
6 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the toxicity of Reaction products of ricinoleic acid with 2-aminoethanol and maleic acid and sodium hydrogensulfite to Desmodesmus subspicatus was determined in a static 72-Hour Algal Growth Inhibition Test. The test was performed according to OECD 201 and EU Method C.3. The nominal concentrations of the test item of 2.5, 8.0, 25, 80 and 249 mg test item/L (corresponding to 1.0, 3.2, 10, 32 and 100 mg solid content/L). The measured concentrations of the test item Reaction products of ricinoleic acid with 2-aminoethanol and maleic acid and sodium hydrogensulfite in the test media of the nominal test concentrations of 2.5 to 249 mg test item/L (corresponding to 1.0 – 100 mg solid content/L) were between 93 to 103% of the nominal values at the start of the test. During the test period of 72 hours, a slight decrease of test item concentration in the test media occurred. At the end of the test, 77 to 96% of the nominal values were found. Since all tested concentrations except the lowest one (77% of nominal at test end) were found to be in the range of 80-120%, the biological results were related to the nominal concentrations. The algal biomass in the samples was determined by measurement of the algal cell density using an electronic particle counter (Coulter Counter, Model Z2). The measurements were performed at least in duplicate.

The cell density was determined at 0, 24, 48 and 72 hours. The growth of the control cultures fulfilled the validity criteria from OECD 201 (2006). The 72 -hour ErC10 and ErC50 are 6.0 and 87 mg solid content/L based on the nominal concentration.

This information is considered to be relevant and reliable for the further risk assessment.