Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2006
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
QAU mentioned, but no GLP Statement. Test above water solubility. Analytical phase report in Chinese. Even though no evaluation of analytical data is possible, results are scientifically reliable. No toxicity occurred and the same result is expected when testing at water solubility limit. Semi-static test design was chosen which guaranteed a relatively constant exposure level.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Soybean oil, epoxidized, reaction products with methanol and water
IUPAC Name:
Soybean oil, epoxidized, reaction products with methanol and water
Details on test material:
The substance was tested by the test house using a trade name that is considered confidential business information. The name assigned to the substance has therefore been changed to Cargill BP-A.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The dissolved oxygen concentration, the pH and temperature in the controls and the test solutions should be measured once daily and at the beginning and the duration of the test.

Test solutions

Vehicle:
yes
Details on test solutions:
Prior to each test medium preparation on Days 0, 1, 2 and 3, the test medium with the nominal concentration of 100 mg/L was prepared by weighting 300 mg of the test substance into 3 L of test water with 300mg Tween-20 solvent.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Brachydanio rerio which was obtained from Amazonas Commercial Aquarium, Shanghai were held in our laboratory for 12 days without any medication. Prior to the test start, they were acclimated for one week under conditions of water quality and illumination similar to those used in the test. During holding and acclimatization until one day before the start of the test the fish were fed with a commercial fish diet. During holding and acclimatization no fish died in the test fish batch and all fish were healthy.
From the acclimated test fish batch, 10 fish were measured at the start of the test: The mean body length of the fish was 2.0±01 cm (Mean ± SD), and the mean body weight was 0.10 ± 0.01 g (Mean ± SD).

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
No data

Test conditions

Hardness:
250 mg CaCo3 per liter
Test temperature:
ca. 23°C
pH:
8.02-8.22
Dissolved oxygen:
>80%
Salinity:
No data
Nominal and measured concentrations:
Nominal concentraion: 100 mg/L
Mean measured concentration: 108 mg/l
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No fish death during the limit test
Results with reference substance (positive control):
The 24h-LC50 of the potassium dichromate (AR.) was calculated as 258 mg/L which fulfilled the criteria for a valid test that the 24h-LC50 of the potassium dichromate should range 200 rnglL to 400 mg/L.
Reported statistics and error estimates:
No data

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The substance had no acute toxic effects on zebra fish under these test conditions.