Soybean oil, epoxidised, reaction products with methanol and water

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

QAU mentioned, but no GLP Statement. Test above water solubility. Analytical phase report in Chinese. Even though no evaluation of analytical data is possible, results are scientifically reliable. No toxicity occurred and the same result is expected when testing at water solubility limit. Semi-static test design was chosen which guaranteed a relatively constant exposure level.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Soybean oil, epoxidized, reaction products with methanol and water

IUPAC Name:

Soybean oil, epoxidized, reaction products with methanol and water

Details on test material:

The substance was tested by the test house using a trade name that is considered confidential business information. The name assigned to the substance has therefore been changed to Cargill BP-A.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The dissolved oxygen concentration, the pH and temperature in the controls and the test solutions should be measured once daily and at the beginning and the duration of the test.

Test solutions

Vehicle:

yes

Details on test solutions:

Prior to each test medium preparation on Days 0, 1, 2 and 3, the test medium with the nominal concentration of 100 mg/L was prepared by weighting 300 mg of the test substance into 3 L of test water with 300mg Tween-20 solvent.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio which was obtained from Amazonas Commercial Aquarium, Shanghai were held in our laboratory for 12 days without any medication. Prior to the test start, they were acclimated for one week under conditions of water quality and illumination similar to those used in the test. During holding and acclimatization until one day before the start of the test the fish were fed with a commercial fish diet. During holding and acclimatization no fish died in the test fish batch and all fish were healthy.
From the acclimated test fish batch, 10 fish were measured at the start of the test: The mean body length of the fish was 2.0±01 cm (Mean ± SD), and the mean body weight was 0.10 ± 0.01 g (Mean ± SD).

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

No data

Test conditions

Hardness:

250 mg CaCo3 per liter

Test temperature:

ca. 23°C

pH:

8.02-8.22

Dissolved oxygen:

>80%

Salinity:

No data

Nominal and measured concentrations:

Nominal concentraion: 100 mg/L
Mean measured concentration: 108 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No fish death during the limit test

Results with reference substance (positive control):

The 24h-LC50 of the potassium dichromate (AR.) was calculated as 258 mg/L which fulfilled the criteria for a valid test that the 24h-LC50 of the potassium dichromate should range 200 rnglL to 400 mg/L.

Reported statistics and error estimates:

No data

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance had no acute toxic effects on zebra fish under these test conditions.