Sodium 1-amino-9,10-dihydro-4-[5-[(2-hydroxyethyl)sulphamoyl]-3,4-xylidino]-9,10-dioxoanthracene-2-sulphonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

other: HPLC-column

Specific details on test material used for the study:

Name: FAT 20033/L
Batch number: AT-PD13-359A1
EAS Test item code: 2014-000179
Appearance / colour: solid / dark blue
CAS number: 25797-81-3
Molecular weight 544.58 g/mol (base) 567.57 g/mol (salt)
Date of analysis: 07 Jan 2014
Expiry date: 07 Jan 2019
Storage conditions: ambient (+5 to +30 °C), dark, dry

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

HPLC-system:
Agilent Technologies 1260 Infinity
Quaternary pump G1311B
Autosampler ALS G1367E
Column oven G1316A
Detector DAD G4212B

Column specification: EC 150/4.6 Nucleodur 100-5 CN-RP 150 x 5
Column temperature: 25 °C
Detection wavelength standard solutions: 220 nm
Detection wavelength test item: 257 nm
Mobile phase: Methanol/water (55/45 ,v/v)
Flow rate: 0.5 mL/min
Injection volume: 2 µL (reference items); 2 µL (test item)

Analytical monitoring:

no

Type:

log Koc

Value:

< 1.5

Temp.:

25 °C

Details on results (HPLC method):

The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (Nucleodur 100-5 CN-RP). A 55/45 (v/v) methanol water mixture was used as mobile phase and the column temperature was 25 °C.

FAT 20033/L eluted with a retention time of 2.45 min. The calculated adsorption coefficient is therefore below the determination limit of the method (<1.5).

Validity criteria fulfilled:

yes

Conclusions:

The Log Koc of FAT 20033/L TE was found to be <1.5.

Executive summary:

The aim of this study was the determination of the adsorption coefficient of the test item FAT 20033/L by high performance liquid chromatography (HPLC) method according to EC method C.19 and OECD guideline 121. The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (Nucleodur 100-5 CN-RP). A55/45 (v/v) methanol water mixture was used as mobile phase and the column temperature was 25 °C.

 

FAT 20033/L eluted with a retention time of 2.45 min. The calculated adsorption coefficient is therefore below the determination limit of the method.

The results are listed below:

 

Peak No.	Mean retention time of two runs [min]	Absolute deviation [min]	Mean log Kocof two runs
1	2.45	0.03	< 1.5 (-3.71)*

*: below determination limit of the method

Description of key information

The Log Koc of FAT 20033/L was found to be <1.5.

Key value for chemical safety assessment

Koc at 20 °C:

31.6

Additional information

The aim of this study was the determination of the adsorption coefficient of the test item FAT 20033/L by high performance liquid chromatography (HPLC) method according to EC method C.19 and OECD guideline 121.

 

FAT 20033/L eluted with a retention time of 2.45 min. The calculated adsorption coefficient is therefore below the determination limit of the method.

 

The results are listed below:

Peak No.	Mean retention time of two runs [min]	Absolute deviation [min]	Mean log Kocof two runs
1	2.45	0.03	< 1.5 (-3.71)*

*: below determination limit of the method

 

[Log Koc: 1.5]