Potassium dimethyldithiocarbamate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-2 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

Weight range was exceeded; occlusive dressing was used

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Potassium dimethyldithiocarbamate

EC Number:

204-875-1

EC Name:

Potassium dimethyldithiocarbamate

Cas Number:

128-03-0

Molecular formula:

C3H7NS2.K

IUPAC Name:

potassium (dimethylcarbamothioyl)sulfanide

Details on test material:

- Name of test material (as cited in study report): Busan 85
- Physical state: Green liquid
- Composition of test material, percentage of components: 52.11% KDDC
- Lot/batch No.: 4-10129
- Stability under test conditions: guaranteed by the sponsor

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Tarvins Rabbitree, Jacksonville, Arkansas, USA
- Age at study initiation: 2-5 months
- Weight at study initiation: 2.0-3.5 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- % coverage: 10
- Type of wrap if used: occlusive


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.65, 1.62, 2.57, 3.24 mL/kg bw
- Concentration (if solution): undiluted
- Constant volume or concentration used: no

Duration of exposure:

24 h

Doses:

794, 1995, 3162, 3981 mg/kg bw

No. of animals per sex per dose:

5 per sex (low dose: 3 female/7 male)

Control animals:

not required

Details on study design:

Observations for death or overt signs of toxicity were made in 2 hours-intervals on the day of dosing, then once daily for fourteen days
Body weights: prior to application of the test material on Day 0 and on Days 7 and 14.
Necropsy: at scheduled sacrifice

Statistics:

LD50 was determined using Statistical Analysis System (SAS) PROBIT procedure

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

2 990 mg/kg bw

95% CL:

2 081 - 4 111

Effect levels 2

Sex:

female

Dose descriptor:

LD0

Effect level:

>= 3 162

Mortality:

794 mg/kg: 0/7(m), 0/3(f)
1995 mg/kg: 1/5(m), 0/5(f)
3162 mg/kg: 3/5(m), 0/5(f)
3981 mg/kg: 4/5(m)

Clinical signs:

other: Signs of systemic toxicity noted were (in decreasing order of incidence): rhinorrhea, yellow nasal discharge, ocular and/or oral discharge, loose stool/diarrhea, salivation, dyspnea/wheezing, lacrimation, lethargy, emaciation. Less commonly were ataxia, p

Gross pathology:

No treatment-related abnormalities were noted.

Any other information on results incl. tables

Table A6_1_2-1.             Mortality after administration of Busan 85
Dose [mg/kg]	Volume Dosage [ml/kg]	Mortality
		Male	Female
794	0.65	0/7		0/3
1995	1.62	1/5		0/5
3162	2.57	3/5		0/5
3981	3.24	4/5		---
LD50 [mg/kg]		2990		>3162