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Diss Factsheets

Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Activities of hepatic epoxide hydrolase and glutathione-S-transferase under influence of TMD, TMTM of dimethyl dithiocarbamate
Author:
Schreiner, E. & Freundt, K.J.
Year:
1985
Bibliographic source:
Toxicol.| Letters, 25, 147-152.

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no
Type of method:
other: in vitro and in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetramethylthiuram monosulphide
EC Number:
202-605-7
EC Name:
Tetramethylthiuram monosulphide
Cas Number:
97-74-5
Molecular formula:
C6H12N2S3
IUPAC Name:
tetramethylthiuram monosulphide
Details on test material:
no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
female

Administration / exposure

Route of administration:
oral: gavage
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
one administration
Frequency of treatment:
one administration
Post exposure period:
18-hours
Doses / concentrations
Remarks:
Doses / Concentrations:
up to 4.16 mmol/kg
Basis:
actual ingested
No. of animals per sex per dose:
no data

Examinations

Examinations:
The epoxide hydrolase activity, the glutathione-S-transferase activity in the cytosol and microsomes of the liver were measured.
Positive control:
no

Results and discussion

Details on results:
The epoxide hydrolase activity in the liver of adult female  Wistar significantly increase 18 hours after the administration by gavage of TMTM (from 2.08 mmol/kg).  The glutathione-S-transferase activity in the cytosol and microsomes of the liver was slightly enhanced after oral administration of TMTM (up to 4.16 mmol/kg). In vitro, TMTM significantly enhanced the hepatic activity of epoxide hydrolase prapared from adult rat.

Applicant's summary and conclusion

Executive summary:

The epoxide hydrolase activity in the liver of adult female Wistar significantly increase 18 hours after the administration by gavage of TMTM (from 2.08 mmol/kg).  The glutathione-S-transferase activity in the cytosol and microsomes of the liver was slightly enhanced after oral administration of TMTM (up to 4.16 mmol/kg). In vitro, TMTM significantly enhanced the hepatic activity of epoxide hydrolase prapared from adult rat.

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