Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Activities of hepatic epoxide hydrolase and glutathione-S-transferase under influence of TMD, TMTM of dimethyl dithiocarbamate
Author:
Schreiner, E. & Freundt, K.J.
Year:
1985
Bibliographic source:
Toxicol.| Letters, 25, 147-152.

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no
Type of method:
other: in vitro and in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetramethylthiuram monosulphide
EC Number:
202-605-7
EC Name:
Tetramethylthiuram monosulphide
Cas Number:
97-74-5
Molecular formula:
C6H12N2S3
IUPAC Name:
N,N-dimethyl[(dimethylcarbamothioyl)sulfanyl]carbothioamide
Details on test material:
no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
female

Administration / exposure

Route of administration:
oral: gavage
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
one administration
Frequency of treatment:
one administration
Post exposure period:
18-hours
Doses / concentrations
Remarks:
Doses / Concentrations:
up to 4.16 mmol/kg
Basis:
actual ingested
No. of animals per sex per dose:
no data

Examinations

Examinations:
The epoxide hydrolase activity, the glutathione-S-transferase activity in the cytosol and microsomes of the liver were measured.
Positive control:
no

Results and discussion

Details on results:
The epoxide hydrolase activity in the liver of adult female  Wistar significantly increase 18 hours after the administration by gavage of TMTM (from 2.08 mmol/kg).  The glutathione-S-transferase activity in the cytosol and microsomes of the liver was slightly enhanced after oral administration of TMTM (up to 4.16 mmol/kg). In vitro, TMTM significantly enhanced the hepatic activity of epoxide hydrolase prapared from adult rat.

Applicant's summary and conclusion

Executive summary:

The epoxide hydrolase activity in the liver of adult female Wistar significantly increase 18 hours after the administration by gavage of TMTM (from 2.08 mmol/kg).  The glutathione-S-transferase activity in the cytosol and microsomes of the liver was slightly enhanced after oral administration of TMTM (up to 4.16 mmol/kg). In vitro, TMTM significantly enhanced the hepatic activity of epoxide hydrolase prapared from adult rat.