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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980
Reference Type:
publication
Title:
Acute Toxicologic Evalutation of Tetramethyl thiuram monosulphide
Author:
Bombard E, Löser E
Year:
1996
Bibliographic source:
J.Am.Coll.Toxicol., Vol 15, Suppl 1

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
no
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
tetramethylthiuram monosulfide
IUPAC Name:
tetramethylthiuram monosulfide
Constituent 2
Chemical structure
Reference substance name:
Tetramethylthiuram monosulphide
EC Number:
202-605-7
EC Name:
Tetramethylthiuram monosulphide
Cas Number:
97-74-5
Molecular formula:
C6H12N2S3
IUPAC Name:
tetramethylthiuram monosulphide
Test material form:
solid: particulate/powder

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: W.Ostrop/Meerbusch
- Age at study initiation: no data
- Weight at study initiation: 2.9 kg
- Housing: individually
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7-14 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18 °C
- Humidity (%): 40%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
100 µl
Duration of treatment / exposure:
24h
Observation period (in vivo):
24-48-72h, 8 days
Number of animals or in vitro replicates:
6 rabbits
Details on study design:
REMOVAL OF TEST SUBSTANCE : no
SCORING SYSTEM: Draize score
TOOL USED TO ASSESS SCORE: fluorescein

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
6 rabbits
Time point:
24/48/72 h
Score:
0
Max. score:
4
Irritation parameter:
iris score
Basis:
mean
Remarks:
6 rabbits
Time point:
24/48/72 h
Score:
0
Max. score:
2
Irritation parameter:
conjunctivae score
Remarks:
(redness)
Basis:
mean
Remarks:
6 rabbits
Time point:
24/48/72 h
Score:
0.44
Max. score:
3
Reversibility:
fully reversible within: 8 days
Remarks on result:
other: Individual scores = 1-0-0.33-0.33-0 -1
Irritation parameter:
chemosis score
Basis:
mean
Remarks:
6 rabbits
Time point:
24/48/72 h
Score:
0.22
Max. score:
4
Reversibility:
fully reversible within: 72 hours
Remarks on result:
other: Individual scores = 0.33-0-0-0.33-0-0.66
Irritant / corrosive response data:
No effect was observed on cornea and iris. Very slight effects on conjunctivae were observed, and are fully reversible in 8 days.
Other effects:
no

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Remarks:
Not eye irritating
Conclusions:
According to these results, TMTM was considered as not irritating for eyes in rabbits.
Executive summary:

Tetramethylthiuram monosulfide was placed in the conjunctival sac of one eye of each of six albino rabbits. The irritant reactions were scored until complete recovery. Some of the instilled powder was retained within the eye for at least 24 hours.

No effect was observed on cornea and iris. Very slight effects on conjunctivae were observed, and are fully reversible in 8 days. According to these results, TMTM was considered as not irritating for eyes in rabbits.

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