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EC number: 271-178-7 | CAS number: 68516-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable to guideline study with acceptable restrictions (limited details of study protocol, only plate incorporation protocol [preincubation is recommended for azo-dyes], no GLP; purity not specified)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report Date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only plate incorporation protocol, limited documentation
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Analytical purity: commercial grade
- Lot/batch No.: Kores sample TS 884
- Stability under test conditions: ensured
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 102 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- 1 ml activation mixture contains: 0.3 ml S9 fraction of liver from rats induced with Aroclor 1254 and 0.7 ml of a solution of co-factors
- Test concentrations with justification for top dose:
- First experiment; preliminary toxicity test: 0.08 - 5000 µg/plate
Second experiment; main experiment: 20, 78, 313, 1250 and 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO (suspension)
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9-mix: 5 and 10 µg DR for TA 98; 0.125 and 0.25 µg 4-NQO for TA 100; 0.5 and 1.0 µg MMC for TA 102; 2.5 and 5.0 µg SA for TA 1535; for 50 and 100 µg 9AA for TA 1537.
- Remarks:
- Positive control substances without S9-mix (continue): : 5 µg 2-AA for TA 98, TA 100 and TA 1537; 20 µg 2-AA for TA 102 and 250 µg CCP for TA 1535.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation), in triplicates
DURATION
- Exposure duration: 48 hours at 37 °C
SELECTION AGENT (mutation assays): histidine deprivation
DETERMINATION OF CYTOTOXICITY
- Method: cloning efficiency - Evaluation criteria:
- Doubling of the colony number is given as a cut-off value in the report.
- Statistics:
- When the colonies had been counted, the arithmetic mean was calculated.
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 102 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- no increase in the number of revertants in test groups in comparison with the negative control treatment was observed
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- a growth-inhibiting effect of the test substance was observed with strain TA 102 in the experiments without and with microsomal activation at the highest concentration.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: at the concentrations of 78µg/plate and above, the substance precipitated in soft agar. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Salmonella/mammalian-microsome mutagenicity test
Metabolic activation | Test substance concentration (µg/plate) | Number of revertants per plate (arithmetic mean) | ||||
TA 98 | TA 100 | TA 102 | TA 1535 | TA 1537 | ||
Without S9-mix | Negative control (DMSO) | 23 | 143 | 261 | 14 | 10 |
20 µg/plate | 30 | 138 | 270 | 16 | 10 | |
78 µg/plate | 25 | 136 | 260 | 19 | 8 | |
313 µg/plate | 22 | 128 | 244 | 14 | 6 | |
1250 µg/plate | 25 | 132 | 288 | 13 | 8 | |
5000 µg/plate | 30 | 125 | 192 | 12 | 5 | |
Corresponding positive control | 450-758 | 563-1149 | 694-1090 | 746-869 | 31-646 | |
With S9-mix | Negative control (DMSO) | 50 | 142 | 232 | 9 | 15 |
20 µg/plate | 49 | 138 | 339 | 13 | 20 | |
78 µg/plate | 48 | 140 | 364 | 9 | 15 | |
313 µg/plate | 47 | 123 | 312 | 11 | 7 | |
1250 µg/plate | 56 | 133 | 306 | 15 | 12 | |
5000 µg/plate | 53 | 109 | 87 | 17 | 9 | |
Corresponding positive control | 963 | 1266 | 1485 | 482 | 63 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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