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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
other: HPLC-column
Specific details on test material used for the study:
Name: FAT 36014/Z
EAS Test item code: 2014-000181
Chemical name: 3-Nitro-n-phenyl-4-(phenylamino)benzene sulfamide
CAS number: 5124-25-4
Density: not applicable
Date of analysis: 07 Aug 2013
Batch number: Q30661AASY
Appearance / colour: powder / yellow
Purity analysed: 94 % w/w
Molecular weight: 369.4 g/mol
Expiry date: 08 Aug 2018
Storage conditions: ambient, dark, dry
Radiolabelling:
no
Test temperature:
25 °C
Details on study design: HPLC method:
HPLC-system:
Agilent Technologies 1260 Infinity
Quaternary pump G1311B
Autosampler ALS G1367E
Column oven G1316A
Detector DAD G4212B
Column specification: EC 150/4.6 Nucleodur 100-5 CN-RP 150 x 5
Column temperature: 25 °C
Detection wavelength standard solutions: 220 nm
Detection wavelength test item: 220 nm
Mobile phase: Methanol/water (55/45 v/v)
Flow rate: 0.5 mL/min
Injection volume: 2 µL (reference items); 2 µL (test item)
Analytical monitoring:
not specified
Details on sampling:
None
Details on matrix:
None
Details on test conditions:
None
Type:
log Koc
Value:
4.14 dimensionless
Temp.:
25 °C
Details on results (HPLC method):
The aim of this study was the determination of the adsorption coefficient of the test item FAT 36014/Z by high performance liquid chromatography (HPLC) method according to EC method C.19 and OECD guideline 121. The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (Nucleodur 100-5 CN-RP). A 55/45 (v/v) methanol/ water mixture was used as mobile phase and the column temperature was 25 °C. FAT 36014/Z eluted with a retention time of 13.43 min and therefore after the highest reference item phenanthrene. Hence the reference item DDT was used as highest standard.
Adsorption and desorption constants:
no data
Recovery of test material:
no data
Concentration of test substance at end of adsorption equilibration period:
no data
Concentration of test substance at end of desorption equilibration period:
no data
Details on results (Batch equilibrium method):
no data
Statistics:
no data
Validity criteria fulfilled:
yes
Conclusions:
The adsorption coefficient (log Koc) was found to be 4.14 at 25 °C temperature.
Executive summary:

The aim of this study was the determination of the adsorption coefficient of the test item FAT 36014/Z by high performance liquid chromatography (HPLC) method according to EC method C.19 and OECD guideline 121. The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. A moderately polar stationary phase based on a silica matrix was used (Nucleodur 100-5 CN-RP). A 55/45 (v/v) methanol/ water mixture was used as mobile phase and the column temperature was 25 °C. FAT 36014/Z eluted with a retention time of 13.43 min and therefore after the highest reference item phenanthrene. Hence the reference item DDT was used as highest standard. Based on the findings of the study, the adsorption coefficient (log Koc) was found to be 4.14 at 25 °C temperature.

Description of key information

A study was performed with a aim for determination of the adsorption coefficient of the test item FAT 36014/Z by high performance liquid chromatography (HPLC) method according to EC method C.19 and OECD guideline 121.The analysis was performed on an analytical column packed with a commercially available cyanopropyl solid phase containing lipophilic and polar moieties. FAT 36014/Z eluted with a retention time of 13.43 min and after the highest reference item. Based on the elution profile the adsorption coefficient (log Koc) was found to be 4.14 at 25 °C temperature.

Key value for chemical safety assessment

Koc at 20 °C:
13 804

Additional information