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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no
Specific details on test material used for the study:
Test material: FAT 36034/B
Analytical monitoring:
no
Details on sampling:
no data
Details on test solutions:
no data
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow trout: (Salmo gairdneri)
Replicates: 8 per test concentration
Age: about 4-6 months
Size: 4-8 cm
Weight: 3-6 g
Supplier: P. Hohler, 4314 Zeiningen
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
no data
Test temperature:
14 °C
pH:
7.6 - 8.3
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
100, 200 and 1000 mg/L
Details on test conditions:
Refer "Any other information on materials and methods incl. tables"
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
ca. 40 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
450 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
ca. 90 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
ca. 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Details on results:
No fish died at 100 and 200 mg/L while all fish died at 1000 mg/L.
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
no data
Sublethal observations / clinical signs:

Concentration
(mg/L)		 Surviving fish pH
24 h 48 h
 100 8 8 7.8
200 8 8 7.6
 1000  0    8.3
Validity criteria fulfilled:
not specified
Conclusions:
The 48 h LC0 for rainbow trout (Onchorhyncus mykiss) was 200 mg/L (i.e. ca. 40 mg a.i./L), the 48 h LC50 was 450 mg/L (i.e. ca. 90 mg a.i./L) and the 48 h LC100 was 1000 mg/L (i.e. ca. 200 mg/L).
Executive summary:

A study was conducted to determine the acute toxicity of the test substance (at ca. 50 % purity) to rainbow trout (Onchorhyncus mykiss). Groups of 8 fish were exposed at 100, 200 or 1000 mg/L for a duration of 48 h. The number of surviving fish was recorded at 24 and 48 h. No fish died at 100 and 200 mg/L while all fish died at 1000 mg/L. Under the test conditions, the 48 h LC0 was 200 mg/L (i.e. ca. 40 mg a.i./L), the 48 h LC50 was 450 mg/L (i.e. ca. 90 mg a.i./L) and the 48 h LC100 was 1000 mg/L (i.e. ca. 200 mg/L).

Description of key information

A study was conducted to determine the acute toxicity of the test substance (at ca. 20 % purity) to rainbow trout (Onchorhyncus mykiss). Groups of 8 fish were exposed at 100, 200 or 1000 mg/L for a duration of 48 h. The number of surviving fish was recorded at 24 and 48 h. No fish died in any treatment group. Under the test conditions, the 48 h LC0 was 200 mg/L (i.e. ca. 40 mg a.i./L), the 48 h LC50 was 450 mg/L (i.e. ca. 90 mg a.i./L) and the 48 h LC100 was 1000 mg/L (i.e. ca. 200 mg/L).


In a supporting study, the LC50 of the test substance (at ca. 40 % purity) was determined to be >1000 mg/L (>400 mg a.i./L) as no mortality was seen in this study.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
90 mg/L

Additional information