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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, GLP
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
Commission Regulation (EC) No 440/2008, Annex, Part C, C.1.: "Acute Toxicity for Fish"
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0(control), 80, 36, 17, 7.5 and 3.4 mg test item/L corresponding to following geometric mean measured concentrations of the test item: 79.20, 33.48, 15.47, 6.53 and 2.79 mg test item/L

- Sampling method: concentrations checked at test start + in 96 h old medium However, from the test concentrations of 80 mg test item/L and 36 mg test item/L and duplicate samples were taken after 24 and 72 hours, respectively, because all fish were dead.
All test medium samples were taken from the approximate centre of the aquaria.

- Sample storage conditions before analysis: no data

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: substance was weighed directly
- Controls: purified drinking water
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
- Species: Rainbow trout (Oncorhynchus mykiss)
- Age and Size: Juveniles;
- Mean body length of the fish: 4.83 cm ± 0.3 cm (Mean ± SD)
- Mean body wet weight 1.12 ± 0.23 g (Mean ± SD).
- Sex: Male and female
- Origin: The test fish were obtained from Forellenzuchtbetrieb Störk, 88348
Bad Saulgau, Germany
- Holding Conditions: All fish were obtained and held in the laboratory for at least 12 days
before the start of the test. They were held in water of the quality to be used in the test for at least seven days immediately before testing under the following conditions:
- Light: 12 - 16 hours photoperiod daily
- Temperature: 13 - 17 °C
- Oxygen concentration: at least 80 % of the air saturation value
- Feeding: three times per week or daily until 24 hours before the test was started
During the last 7 days prior to the start of the test no fish (0%) died in the test fish batch. Therefore the mortalities in the fish batch were below 5 % and the fish batch was accepted.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no
Hardness:
2.5 mmol/L (= 250.0 mg/L) as CaCO3
Test temperature:
14 to 15 °C;
pH:
7.8 to 8.1
Dissolved oxygen:
90 to 100%
Salinity:
CaCl2 × 2H2O : 2.0 mmol/L (= 294.0 mg/L)
MgSO4 × 7H2O : 0.5 mmol/L (= 123.0 mg/L)
NaHCO3 : 0.75 mmol/L (= 65.0 mg/L)
KCl : 0.075 mmol/L (= 5.8 mg/L)
Ratio of
Ca : Mg = 4 : 1 (based on molarity)
Na : K = 10 : 1 (based on molarity)
Nominal and measured concentrations:
0(control), 80, 36, 17, 7.5 and 3.4 mg test item/L corresponding to following geometric mean measured concentrations of the test item: 79.20, 33.48, 15.47, 6.53 and 2.79 mg test item/L
Details on test conditions:
- Test vessel
- Material, size, headspace, fill volume: 12 L all-glass aquaria, 10 L fill volume
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): daily renewal
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
In deionised water (conductivity ≤ 10 μScm-1) analytical grade salts were added to following nominal concentrations:
CaCl2 × 2H2O : 2.0 mmol/L (= 294.0 mg/L)
MgSO4 × 7H2O : 0.5 mmol/L (= 123.0 mg/L)
NaHCO3 : 0.75 mmol/L (= 65.0 mg/L)
KCl : 0.075 mmol/L (= 5.8 mg/L)
Water Hardness : 2.5 mmol/L (= 250.0 mg/L) as CaCO3
Alkalinity : 0.8 mmol/L
Ratio of Ca : Mg = 4 : 1 (based on molarity)
Na : K = 10 : 1 (based on molarity)


OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 450 to 1110 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, abnormal behaviour (daily observation); dead animals were removed from the test vessels

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
36.54 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: n.d.
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
32.58 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: n.d.
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
22.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 17.83-28.33 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
22.36 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 17.83-28.33 mg/l
Details on results:
- Mortality of control: 0/10 died
Results with reference substance (positive control):
not applicaable
Reported statistics and error estimates:
ToxRat Professional, Version 2.10.05, ToxRat Solutions GmbH.

Observed mortality of unfed Rainbow troute (Oncorhynchus mykiss) exposed to Butyldiglycol methacrylate

Mortality

Geometric mean concentration

[mg/l]

Exposure time [h]

mg/l

0

2

24

48

72

96

 

# mort

# mort

# mort

# mort

# mort

# mort

Control

0

0

0

0

0

0

2.79

0

0

0

0

0

0

6.53

0

0

0

0

0

0

15.47

0

0

0

0

0

0

33.48

0

0

2

4

7

7

79.20

0

0

7

7

7

7

LC50 [mg/l]

-

n.d

36.54

32.58

22.36

22.36

95% CI.

-

n.d

n.d.

n.d.

17.83-28.33

17.83-28.33

Validity criteria fulfilled:
yes
Conclusions:
Based on the test results the 96-hour LC50 of Butyldiglycol methacrylate for rainbow trout (Oncorhynchus mykiss) was determined to be 22.36 mg test item/L based on geometric mean concentrations. The LC0 was determined to be 15.47 mg test item/L also based on geometric mean
concentrations.
Executive summary:

In a 96-h acute toxicity study according to OECD guideline 203, adopted 17th July 1992, Onchorynchus mykiss (Rainbow troute) were exposed to BDGMA at nominal concentrations of 0 (control), 80, 36, 17, 7.5 and 3.4 mg test item/L corresponding to geometric mean measured concentrations of  79.20, 33.48, 15.47, 6.53 and 2.79 mg test item/L under semistatic conditions.

The 96-h LC50 was 22.36 mg/L (based on geometric mean measured concentration).

This toxicity study is classified as reliable without restriction and satisfies the guideline requirement for acute toxicity study to fish.

Results Synopsis

Test organism : mean length 4.83 cm ± 0.3 cm, mean weight 1.12 ± 0.23 g

Test Type: semistatic

LC50: 22.36 mgL 95% C.I.: 17.83-28.33 mg/L

LCo: 15.47 mg/L

Endpoint(s) Effected: mortality

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

The 96-h LC50 of BDGMA in freshwater fish was 22.36 mg/l (OECD 203, semistatic). 

Key value for chemical safety assessment

LC50 for freshwater fish:
22.36 mg/L

Additional information

One acute freshwater study to fish is available for BDGMA, which is considered reliable without restriction (Klimisch score 1).

In a 96-h acute toxicity study according to OECD 203, rainbow troute (onchorynchus mykiss) were exposed to BDGMA at nominal concentrations of 0 (control), 80, 36, 17, 7.5 and 3.4 mg test item/L corresponding to geometric mean measured concentrations of 79.20, 33.48, 15.47, 6.53 and 2.79 mg test item/L under semistatic conditions.

 

The 96-h LC50 was 22.36 mg/L (based on geometric mean measured concentration).