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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08.08.2005-15.11.2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, GLP
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
13th April 2004
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
A stock solution with 400 mg Butyldiglycol methacrylate per litre dilution water was prepared.
• Each test concentration was prepared in a volumetrie flask (500 mL) by dilution of appropriate amounts of the stock solution.
• All solutions were stirred continuously for homogeneity.

Concentration of test
substance
[mg/L] to be tested Amount of stock solution in mL pipetted ad 500mL
1 1.25
2 2.5
4 5
8 10
16 20
32 40
64 80
128 160
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: Landesamt für Wasser-und Abfallwirtschaft, Düsseldorf, Germany
- Age: not older than 24 hours
-Number of animals: 30
- Food type: Desmodesmus subspicatus
- Amount: During holding daphnia were fed daily with an appropriate amount of algae which could roughly consumed per day.
-Breeding
Organisms were held in beakers with a nominal capacity of 2000 mL which were filled with approx. 1400 mL dilution water. 15 - 20 adult
organisms were held in one beaker in order to get a high reproduction rate and viable newborns.
Breeding beakers were kept at an additional artificial light-dark cycle of 16 to 8 hours in a temperature controlled laboratory at 20 ± 2°C. Breeding
water was renewed three times a week (Monday, Tuesday, Friday).


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
Total hardness:2.574 mmol/l
Carbonate hardness: 0.7 up to 0.9 mmol/l
Test temperature:
21.7 -22.0 °C
pH:
8.32-8.45
Dissolved oxygen:
Oxygen saturation: 62-67 %
Nominal and measured concentrations:
The nominal concentrations did not deviate from the analytically determined concentrations of the test substance.
The analytically determined amount of the test substance after 0, 24 and 48 hours was 86 % to 99 % of the nominal concentrations.
The analytical determination showed that there was no loss of test substance during the course of the test (48 hours).
Details on test conditions:
Five test beakers were filled with approximately 30 mL of each test concentration and the dilution water (control). For each test concentration and the control one additional beaker was filled for measurement of temperature, oxygen saturation and pH - value.
Temperature and pH value were measured concurrently by pH meter .

•Thirty daphnia, divided into six repetitions were used for each test concentration and the control, respectively, (five per vessel). One of these replicates only served for measurement of temperature, oxygen and pH-value (swimming inability data from these replicates were excluded from evaluation).
• The daphnia were placed in the beakers by means of a small sieve made of stainless steel in order to minimise the transfer of dilution water.
• One and two days after exposure daphnia were observed for immobilisation.
• Daphnia which were unable to swim after gentle agitation of the beaker were considered to be immobile.
• Temperature, oxygen saturation and pH-value were measured daily in each tested concentration and the control.
• The following four additional samples in 400 mL beakers were prepared for anaIytical determinations by the test site AQura GmbH, Marl, Germany. Approximately 300 mL each of the control (dilution water) and the concentrations 1, 16, 128 mg Butyldiglycol methacrylate /L dilution water were
filled into the beakers (to be stored under the same conditions as the test vessels) and an amount of 30 mL was daily (0, 24, 48 h) filled into 50 mL
glass flasks, closed with screw caps and stored in a freezer (approx. -20°C). At the end of the experiment the samples were sent to the test site. For
transport the samples were shipped on ice.
30 ml of the stock solution (400 mg Butyldiglycol methacrylate /L dilution water) were prepared Iikewise on day 0.
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
94.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limits: 68 to 158 mg/l
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
>= 128 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The results of this study show that the test substance Butyldiglycol methacrylate induced swimming inability to the exposed Daphnia within 48 hours.
The ECo, EC5Q and EC100 values after 2 days of exposure were 8 mg/L, 94.7 mg/L and >128 mg/L, respectively.
There were no immobilised Daphnia in the control group.

Vessel No.

Nominal conc

 (mg test substance/l)

Immobilized daphnia

per test vessel

 

 

1d

2d

1-5

Control

0

0

0

0

0

0

0

0

0

0

6-10

1

0

0

0

0

0

0

0

0

0

0

11-15

2

0

0

0

0

0

0

0

0

0

0

16-20

4

0

0

0

0

0

0

0

0

0

0

21-25

8

0

0

0

0

0

0

0

0

0

0

26-30

16

0

0

0

0

0

0

0

0

2

1

31-35

32

0

0

0

0

0

0

1

0

2

1

36-40

64

0

0

0

0

0

0

3

1

1

1

41-45

128

0

0

0

0

0

5

2

3

2

5

 

Nominal conc.

(mg test substance/l)

Number immobilized daphnia

Cumulative frequency (%)

1d

2d

1d

2d

Control

0

0

0

0

1

0

0

0

0

2

0

0

0

0

4

0

0

0

0

8

0

0

0

0

16

0

3

0

12

32

0

4

0

16

64

0

6

0

24

128

0

17

0

68

 

Validity criteria fulfilled:
yes
Conclusions:
EC50 (48 h) Daphnia magna of Butyldigylcol methacrylate is 94.7 mg/l
Executive summary:

The 48–hr-acute toxicity of Butyldiglycol methacrylate to Daphnia magna was studied under static conditions acc. OECD Guideline 202.  Daphnids were exposed to nominal concentration of 1; 2; 4; 8; 16; 32; 64; and 128 mg /L for 48 hr.  Immobilization effects were observed daily.  The 48– hour EC50was 94.7 mg /L.  This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Results Synopsis

Daphnia magna, static, 48 h: 

EC50:  94.7 mg /L      95% C.I. : 68 to 158 mg./L

EC0: 8.mg /L                      

Endpoint effected: mobility

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

EC50 Daphnia magna (48h) = 94.7 mg/l

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
94.7 mg/L

Additional information

One short term study to aquatic invertebrates with BDGMA is available acc. OECD 202 under static conditions which is considered to be reliable without restriction (Klimisch score 1). Daphnids were exposed at nominal concentrations of 1; 2; 4; 8; 16; 32; 64; and 128 mg /L for 48 hr. 

The 48– hour EC50 was 94.7 mg /L.