p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 301D with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

EC Number:

221-453-2

EC Name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Cas Number:

3101-60-8

Molecular formula:

C13H18O2

IUPAC Name:

2-[(4-tert-butylphenoxy)methyl]oxirane

Constituent 2

Reference substance name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

IUPAC Name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

Constituent 3

Reference substance name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

IUPAC Name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

Test material form:

other: Liquid at room temperature.

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. and 4.1.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

A sample of activated sludge (domestic) was acquired from the Brazos River Valley Sewer Authority

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

A mineral salts medium was prepared in deionized water and aerated for approximately 19 hours. Minerasalts media was used as a blank, and homogenized for approximately 30 minutes. The test substance (1.5 mg) was applied to aluminum foil and placed in each replicate, resulting in a dose rate of approximately 5 mg/L. The bottles were then filled to a total volume of 300 mL with the prepared mineral salts media. For the Toxicity Reference Solutions 1.5 mg of the test substance was applied to aluminum foil and placed in each replicate. The bottles were then filled to a total volume of 300 mL with the prepared reference solution.

Ten labeled Biologic Oxygen Demand (BOD) bottles were prepared for the blank, 5 mg/L test substance and reference solutions (up to 5 sampling periods). Only six bottles were prepared for the toxicity reference solution. The blank and reference solutions were added to the respective bottles by siphoning from the lower quarter (not from the bottom) of the prepared solution containers. The Day 0 samples were examined for dissolved oxygen and the remaining bottles were stored in the dark and incubated at 22-23 C. Two BOD bottles for each solution were removed at each sampling interval of 0, 7, 14, 21 and 28 days.

Reference substance

Reference substance:

aniline

Remarks:

2.0 mg/L

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 1.1

Sampling time:

28 d

Remarks on result:

other: Maximum % biodegradation was observed at 28 days of contact.

Details on results:

The % biodegradation of the test substance (5.0 mg/L)
________________________________________________________________
Sampling Days 7 14 21 28

% degradation 0.4 0.0 1.0 1.1

BOD5 / COD results

Results with reference substance:

The Reference substance 2.0 mg/L distilled aniline reached a maximum % biodegradation of 87.2% at 21 days of contact.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance is not readily biodegradable under the conditions of the study.

Executive summary:

The test substance, p-tert-butylphenyl-1-(2,3)-epoxypropyl ether was accessed for potential ready biodegradability in an O.E.C.D. test guideline 301D Closed-Bottle study. The percent Biodegradation of the test substance reached 1.1% at 28 days of sludge contact. Therefore, the test substance is not readily biodegradable under the conditions of the study.