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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was well conducted and described in accordance with GLP standards and according to an OECD guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1997

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
- Substance type: Colourless, clear liquid
- Physical state: liquid
- Analytical purity: 99.8%
- Impurities (identity and concentrations): 0.04% water; less than 0.02% CH3COOH.
- Lot/batch No.:WDK5014
- Stability under test conditions: stable (IR-tested)
- Storage condition of test material: stored in a refrigerator

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- A stock test solution was prepared by adding the test substance to dilution water (c= 10,000 mg/L).
From this stock solution, 4 actual test solutions (for 4 vessels) were prepared by further diluting the solution to 1,000 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: US EPA Environmental research laboratory.
- Age at study initiation : immature
- Method of breeding: 2-4 week old, subcultured daphinas were used as parent for the immatures used in this test.
- Feeding during test:no
- Food type: unicellular algae
- Amount: 0.1 - 0.2 mg carbon/day/water flea.
- Frequency: daily during preparation phase. No feeding during test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
55.6
Test temperature:
20°C +/- 1°C
pH:
7.4 - 8.0
Dissolved oxygen:
8.4 - 8.8 mg/L
Nominal and measured concentrations:
nominal: 1,000 mg/L.

measured:
start of test: 104% of nominal value
end of test: 101% of nominal value
Details on test conditions:
TEST SYSTEM
- Test vessel: tall dish
- Type: open
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water from the city of Kurume
- Chlorine: was analyzed and observed to be lower than 0.02 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment, but checking oh pH.
- Light intensity: Room light

TEST CONCENTRATIONS
- Test concentrations: 1,000 mg/L test solution
- Results used to determine the conditions for the definitive study: A 1,000 mg/L test solution was observed to have no harmful effects on daphnia in a preliminary study.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate; EiC50 = 0.283 mg/L after 48h.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility

Any other information on results incl. tables

The total number of immobilized fleas in all test and control vessels was 0.

Applicant's summary and conclusion

Conclusions:
MPD was observed to cause no immobilizing effects on daphnia magnae after 48hrs at a concentration of 1,000 mg/L.
Executive summary:

Toxic effects of MPD were tested towards Daphnia magna in a 48 -hour acute immobilization test. MPD was observed to have no immobilizing effects on Daphnia magna at a concentration of 1,000 mg/L. The NOEC and EiC50 are >1,000 mg/L.