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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study (GLP), detailed documentation not available

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Unnamed
Year:
1993
Report date:
1993
Reference Type:
publication
Title:
Unnamed
Year:
1994
Report date:
1993

Materials and methods

Principles of method if other than guideline:
Method: other: Japanese Guideline for Screening Mutagenicity Testing of Chemicals
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Lactonitrile
EC Number:
201-163-2
EC Name:
Lactonitrile
Cas Number:
78-97-7
Molecular formula:
C3H5NO
IUPAC Name:
2-hydroxypropanenitrile
Details on test material:
2-Hydroxypropanenitrile, commercial
Purity: 92.3 %

Method

Species / strain
Species / strain / cell type:
other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
Metabolic activation:
with and without
Test concentrations with justification for top dose:
S. typhimurium: 4.69, 9.38, 18.75, 37.5, 75, 150 ug/plate, E. coli WP2 uvrA: 75, 150, 300, 600, 1200 or 2400 ug/plate
Details on test system and experimental conditions:
IUCLID4 Type: Ames test

Results and discussion

Test results
Species / strain:
other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks on result:
other: other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Cytotoxicity 
With metabolic activation: 150 pg/plate (TA), 1500 ug/plate (WP2)
Without metabolic activation: 150 ug/plate (TA), 2400 ug/plate (WP2)

Precipitation
negative

Mutagenicity
With metabolic activation: negative for all strains
Without metabolic activation: negative for all strains

Applicant's summary and conclusion