29H,31H-phthalocyanine

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

June, 1984

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Name of test material (as cited in study report): Heliogen Gruen 8730 Pulver;
Batch No: BW137 (Partien 616-619);

Analytical monitoring:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in demineralized water for about 20 h at approximately 20 ± 2 °C. Undissolved test substance was removed by centrifugation. The eluate had a tinge of green. The nominal concentration of the eluate was 125 (mg/l).

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): culture in the Laboratory of Ecology at BASF AG, Ludwigshafen, Germani. Orginally obtained from SAG (Collection of algal cultures in Göttingen)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

23 ± 2 °C

pH:

8.0 - 8.1

Nominal and measured concentrations:

Nominal test concentrations (prepared by dilution from stock solution): control, 6.25, 12.5, 25, 50 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks plugged with gas permeable siliconsponge caps, 250 mL
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 100 mL
- Aeration: no
- Initial cells density: approx. 10000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH:
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, type universal white (e.g. OSRAM L 25), about 60 - 120 µE/(m2.s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: in vivo chlorophyll-a-fluorescence(pulsed excitation with light flashes having a wavelength of 435 nm)
- measurement of fluorescence: after 0, 24, 48 and 72 h - cell counting: after 72 h in a counting chamber (Neubauer improved) in replicate No. 2 of the inoculated control

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

EC-values calculated (linear regression analysis) from the concentration-response relationship.

Inhibition of the algal growth rates after 72 hours:

Nominal concentration [mg/L]	Biomass [%]	Growth Rate [%]
0	0	0
6.25	1.4	-2.1
12.5	11.1	2.4
25	16.1	4.5
50	19.3	4.7
100	28.4	9.1

  

For growth rate and biomass, the EC50 after 72h lies above the water solubility limit.  Due to the test design the raw eluate has to be diluted (8:1:1) due to the addition of algal suspension and enriched medium.

At the highest technically feasible concentration of the eluate (due to the test design, 80% of the water solubility limit) 9.1 % effect on growth rate and 28.4% effect on biomass appeared. The NOEC lies at 1/10 of the water solubility limit for both endpoints

Validity criteria fulfilled:

yes

Description of key information

The substance is not toxic to aquatic algae, with an ErC50 > 100 mg/L and an ErC10 > 100 mg/L.

Additional information

All substances in this category share a similar structure with a molecular weight > 500 g/mol. Based on the high molecular weight and insolubility, the substances in this category will not be able to penetrate biological membranes. Thus, no systemic exposure is expected. This is confirmed by the environmental studies on aquatic algae for one substance in this category: CAS 1328-53-6. The environmental toxicity data can be used for read-across to all substances in this category.

The study on green algae Desmodesmus subspicatusas was performed according to OECD TG 201. The test concentrations were not analytically verified. The 72-h ErC50 and ErC10 were determined to be > 100 mg/L (the highest concentration tested) (BASF 2000, K2).

The determined ErC50 and ErC10 values were significantly higher than the water solubility of the test substance. Based on the available information, it is concluded that all substances in this category are not toxic to aquatic algae with an ErC50 > 100 mg/L and an ErC10 > 100 mg/L.