29H,31H-phthalocyanine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Acute toxicity upon intraperitoneal injection

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

29H,31H-phthalocyanine

EC Number:

209-378-3

EC Name:

29H,31H-phthalocyanine

Cas Number:

574-93-6

Molecular formula:

C32H18N8

IUPAC Name:

29H,31H-phthalocyanine

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material (as cited in study report): Heliogenblau MFA
- Analytical purity: ca. 100 %

Specific details on test material used for the study:

- Name of test material (as cited in study report): Heliogenblau MFA
- Analytical purity: ca. 100 %

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS:
Average body weight at study initiation:
- males: 26 g
- females: 24 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: suspension containing 0.5 % CMC and 1-2 drops Cremophor EL

Details on exposure:

Concentration of the test material in vehicle:
- 20 % (2000 mg/kg bw),

-Amount of test material applied per gavage:
- 10 ml/kg bw for 2000 mg/kg bw

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5 animals per sex

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: directly, 15 min, 30 min, 1 h, 2 h, 4 h and 5 h after injection, then on day 1, 4, 5, 6, 7, 8, 11, 12, 13 and 14 until conclusion.
- Clinical symptoms and body weight were documented.
- Necropsy of survivors were performed. Deceased animals and those sacrificed at the end of the observation period (on day 14 after dosing) were necropsied.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality was observed.

Mortality:

No mortality of 5 males and 5 females per dose was seen.

Clinical signs:

Some symptoms of poisoning (dyspnoea, apathy, staggering, unkempt fur, exsiccosis and a poor general condition) were noted within the first 5 hours after application of the test substance.

Body weight:

The body weight gain was within the normal range, details can be seen in "Remarks on results including tables and figures".

Gross pathology:

Autopsy revealed incorporation of substance in the abdomen.

Any other information on results incl. tables

Table 1: Mean body weight of mice after intraperitoneal application of Heliogengrün D 8730

Dose level [mg/kg bw]	Males		Females
	2000		2000
Day 3	29.2		26.4
Day 7	30.8		27.8
Day 13 34.433.0   Day 13	34.2		28.6