2-aminobenzenesulphonic acid

Endpoint summary

Additional information

Short-term toxicity to fish

No. of studies were reviewed for Short-term toxicity to fish from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below  

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1.	LC50	193.451mg/L	Mortality	96 hrs.	Pimephales promelas	Predicted data from QSAR for target CAS:88-21-1
2.	LC50	100.4 mg/L	Mortality	96 hrs.	Pimephales promelas	Experimental data from Journal of Hydrology for read across CAS:127-57-3
3.	LC50	480 mg/L	Mortality	48 hrs.	Oryzias latipes	Experimental data from MITI Japan for read across CAS:88-44-8

 

Based on the studies summarized in the above table for the target substance 2-aminobenzenesulphonic acid and the read across substance, the endpoint value was found to vary between LC50 = 100.4 mg/L to 193.451mg/L in a 96 hrs and at 48 hrs. LC50 =480 mg/L; on the basis of mortality effect. Based on the above weight of evidence values, it is concluded that the test substance dose not qualifies for the aquatic toxicity. 

Long-term toxicity to fish

According to column I of annex IX, Long-term toxicity testing on fish need not to be conducted as it is already provide as part of Annex VIII requirements. Therefore this study was considered to be waived.

Short-term toxicity to aquatic invertebrates

The lethal concentration (LC50) value of 2-aminobenzenesulphonic acid in aquatic invertebrate (Daphnia Magna) in a 48 hr study was estimated to be 106.623 mg/L. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that 2-aminobenzenesulphonic acid not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

Long-term toxicity to aquatic invertebrates

According to column I of annex IX ; Long-term toxicity testing on invertebrate (species : Daphnia magna) need not to be conducted as it is already provided as a part of Annex VII requirements.Therefore this end point was considered for waiver.

Toxicity to aquatic algae and cyanobacteria

No. of studies were reviewed for toxicity to aquatic algae from reliable sources having Klimisch rating 2 and 4. 

The summary of the results are presented below   

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1.	NOEC	0.3396714mg/L	Growth	72 hrs.	Pseudokirchnerella subcapitata	Predicted data from QSAR for target CAS : 88-21-1
2.	EC50	198.373mg/l	-	96 hrs.	Green Algae	Predicted data from ECOSAR for target CAS : 88-21-1
3.	EC50	170 mg/l	Population	48 hrs.	Desmodesmus subspicatus (reported as Scenedesmus subspicatus)	Experimental data for read across CAS: 615-36-1

 

Based on the studies summarized in the above table for the target substance 2-aminobenzenesulphonic acid and the read across substance, the endpoint value was found to vary between EC50 = 170 mg/l to 198.373mg/l and NOEC =0.3396714mg/L in a 96,72 and 48 hrs.; on the basis of Population and growth effect. Based on the above weight of evidence values, it is concluded that the test substance dose not qualifies for the aquatic toxicity.  

Toxicity to microorganisms

No. of studies were reviewed for toxicity to microorganisms from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below    

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1.	IGC50	219.2835 mg/L	growth inhibition	48 hrs.	Tetrahymena pyriformis	Predicted data from QSAR for target CAS:88-21-1
2.	IGC50	606.07 mg/L	growth inhibition	46 hrs.	Tetrahymena pyriformis	Predicted data from Danish QSAR for target CAS:88-21-1
3.	EC50	114 mg/L	-	5 min.	Photobacterium phosphoreum	Experimental data from water pollution research journal Canada for read across CAS: 121-57-3
4	IC50	533 mg/L	-	-	Saccharomyces cerevisiae	Experimental data from Methods & Findings in Experimental & Clinical Pharmacology for read across CAS: 121-57-3

 

Based on the studies summarized in the above table for the target substance 2-aminobenzenesulphonic acid and read across substance, the endpoint value was found to be EC50 = 114 mg/L in 5 min.; IGC50 = 219.2835 mg/L to 606.07 mg/L in 46 hrs. to 48 hrs. and IC50 =533 mg/L on the basis of growth inhibition effect.