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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14.11.- 12.12.1990
Reliability:
1 (reliable without restriction)
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1991

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
Principles of method if other than guideline:
not relevant
GLP compliance:
yes
Remarks:
statement of compliance

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of [trans-1,1,4,4-Tetramethoxy-2-butene + cis-1,1,4,4-Tetramethoxy-2-butene]
EC Number:
900-110-7
Cas Number:
5370-08-1
Molecular formula:
C8H16O4
IUPAC Name:
Reaction mass of [trans-1,1,4,4-Tetramethoxy-2-butene + cis-1,1,4,4-Tetramethoxy-2-butene]
Details on test material:
- Name of test material (as cited in study report): C4-Diacetale
- Physical state: colourless liquid
- Analytical purity: 97.1%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Details on results:
No further details

Any other information on results incl. tables

No remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
No physico-chemical degradation was observed when the test substance was incubated without inoculum.
Thus, C4-Diacetal may be characterized as: not easily biodegradable.
Executive summary:

The ready biodegradability of C4-Diacetal has been examined using the modified MITI test I. A mixture of activated sludge from a domestic sewage works and from a pilot chemical waste water treatment plant was exposed under controlled conditions to 100 mg/l of the test substance. After an incubation period of 28 days, the BOD/ThOD ratio was calculated to determine the biological degradability. C4-Diacetal shows a ready biodegradability of <1%. Control experiments revealed that the reference substance sodium benzoate was ultimately biodegraded and that the test substance had no toxic effect on the microogranisms in the activated sludge at concentrations >= 100 mg/l. No physico-chemical degradation was observed when the test substance was incubated without inoculum. Thus, C4-Diacetal may be characterized as: not easily biodegradable.

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