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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
6.- 8. 2.1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline (1984) under GLP
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of [trans-1,1,4,4-Tetramethoxy-2-butene + cis-1,1,4,4-Tetramethoxy-2-butene]
EC Number:
900-110-7
Cas Number:
5370-08-1
Molecular formula:
C8H16O4
IUPAC Name:
Reaction mass of [trans-1,1,4,4-Tetramethoxy-2-butene + cis-1,1,4,4-Tetramethoxy-2-butene]
Details on test material:
- Name of test material (as cited in study report): C4-Diacetale
- Physical state: colourless liquid
- Storage condition of test material: In a tightly closed container at room temperature in the dark

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
275 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 241 - 316 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
401 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 324 - 506 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

After 24 hours of exposure the total rate of immobilization of daphnia was 100% at nominally 1000 mg/L, 65% at nominally 560 mg/L and 45% at nominally 320 mg/L. No immobilization of daphnia was seen at or below 180 mg/L. After 48 hours of exposure immobilization of all daphnia exposed was seen at nominal concentrations of 560 mg/L and higher. A t nominally 320 mg/L the total incidence of immobility had increased to 70%. A t nominally 180 mg/L, 1 out of totally twenty daphnia exposed had become immobile. Since a total of 10% immobility is accepted in the untreated control, this can be considered as not biologically significant. No immobilization of daphnia was seen at 100 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present study C4-DIACETAL did not induce biologically significant acute immobilization of Daphnia magna at or below
180 mg/L after 48 hours of exposure (NOEC) with a 48h-EC50 of 275 mg/l (95% confidence interval between 241 and 316 mg/L).
Executive summary:

Acute Toxicity Study in Daphnia magna with C4 -DIACETAL. Daphnia were exposed for a maximum of 48 hours to a concentration range of 100 to 1000 mg/L forming a geometric progression with a factor of 1.8. The test was performed in duplicate with 10 daphnia per vessel. After 24 hours of exposure the total rate of immobilization of daphnia was 100% at nominally 1000 mg/L, 65% at nominally 560 mg/L and 45% at nominally 320 mg/L. No immobilization of daphnia was seen at or below nominally 180 mg/L. After 48 hours of exposure immobilization of all daphnia exposed was seen at nominal concentrations at and above 560 mg/L. At nominally 320 mg/L the total incidence of immobility had increased to 70%. No biologically significant immobilization of daphnia was seen at 100 and 180 mg/L. Analysis of the samples taken at the start of the final test revealed mean actual concentrations of 81%, 78% and 84% relative to the nominal concentrations o f 100, 320 and 1000 mg/L, respectively. During the period before renewal of the test media (24 hours) the actual concentration remained >80% at all three concentrations sampled.

The 24h-EC50 was 401 mg/L with 95% confidence interval ranging from 324 to 506 mg/L (slope = 5.02). The 48h-EC50 was 275 mg/L with 95% confidence interval ranging from 241 to 316 mg/L (slope = 8.62). Finally, the No Observed Effect Concentration for C4-DIACETAL with respect to mobility of Daphnia magna was found to be 180 mg/L after 48 hours of exposure.

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