2-Butene, 1,1,4,4-tetramethoxy-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

6.- 8. 2.1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to OECD guideline (1984) under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

EC Number:

900-110-7

Cas Number:

5370-08-1

Molecular formula:

C8H16O4

IUPAC Name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

Details on test material:

- Name of test material (as cited in study report): C4-Diacetale
- Physical state: colourless liquid
- Storage condition of test material: In a tightly closed container at room temperature in the dark

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

275 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 241 - 316 mg/L

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

401 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 324 - 506 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

After 24 hours of exposure the total rate of immobilization of daphnia was 100% at nominally 1000 mg/L, 65% at nominally 560 mg/L and 45% at nominally 320 mg/L. No immobilization of daphnia was seen at or below 180 mg/L. After 48 hours of exposure immobilization of all daphnia exposed was seen at nominal concentrations of 560 mg/L and higher. A t nominally 320 mg/L the total incidence of immobility had increased to 70%. A t nominally 180 mg/L, 1 out of totally twenty daphnia exposed had become immobile. Since a total of 10% immobility is accepted in the untreated control, this can be considered as not biologically significant. No immobilization of daphnia was seen at 100 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study C4-DIACETAL did not induce biologically significant acute immobilization of Daphnia magna at or below
180 mg/L after 48 hours of exposure (NOEC) with a 48h-EC50 of 275 mg/l (95% confidence interval between 241 and 316 mg/L).

Executive summary:

Acute Toxicity Study in Daphnia magna with C4 -DIACETAL. Daphnia were exposed for a maximum of 48 hours to a concentration range of 100 to 1000 mg/L forming a geometric progression with a factor of 1.8. The test was performed in duplicate with 10 daphnia per vessel. After 24 hours of exposure the total rate of immobilization of daphnia was 100% at nominally 1000 mg/L, 65% at nominally 560 mg/L and 45% at nominally 320 mg/L. No immobilization of daphnia was seen at or below nominally 180 mg/L. After 48 hours of exposure immobilization of all daphnia exposed was seen at nominal concentrations at and above 560 mg/L. At nominally 320 mg/L the total incidence of immobility had increased to 70%. No biologically significant immobilization of daphnia was seen at 100 and 180 mg/L. Analysis of the samples taken at the start of the final test revealed mean actual concentrations of 81%, 78% and 84% relative to the nominal concentrations o f 100, 320 and 1000 mg/L, respectively. During the period before renewal of the test media (24 hours) the actual concentration remained >80% at all three concentrations sampled.

The 24h-EC50 was 401 mg/L with 95% confidence interval ranging from 324 to 506 mg/L (slope = 5.02). The 48h-EC50 was 275 mg/L with 95% confidence interval ranging from 241 to 316 mg/L (slope = 8.62). Finally, the No Observed Effect Concentration for C4-DIACETAL with respect to mobility of Daphnia magna was found to be 180 mg/L after 48 hours of exposure.