Registration Dossier
Registration Dossier
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EC number: 201-475-9 | CAS number: 83-42-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-chloro-6-nitrotoluene
- EC Number:
- 201-475-9
- EC Name:
- 2-chloro-6-nitrotoluene
- Cas Number:
- 83-42-1
- Molecular formula:
- C7H6ClNO2
- IUPAC Name:
- 1-chloro-2-methyl-3-nitrobenzene
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat Liver
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 500 µg/Plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 500 µg/Plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
At 500 µg/Plate 2 -chloro-6 -nitrotoluene was found to be toxic. Without metabolic activation, the substance was not mutagenic. With activation mutagenicity was found with TA 100 and eventually with TA 98.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
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