Sulfonic acids, C6-8-alkane, sodium salts

Endpoint summary

Description of key information

Acute toxicity to fish: 96-h LC50 > 100 mg/L (Danio rerio; OECD TG 203)

Acute toxicity to aquatic invertebrates: 48-h EC50 > 100 mg/L (Daphnia magna; OECD TG 202)

Toxicity to aquatic algae: 72-h ErC50 > 100 mg/L, NOErC 6.25 mg/L (Pseudokirchneriella subcapitata; OECD TG 201)

Additional information

Acute toxicity to fish: The acute toxicity of the test item to fish as assessed in a study performed according to OECD TG 203 over a period of 96 hours in a static test system using Danio rerio (Citoxlab, 2013).

Based on the results of a preliminary study, the test was performed as a limit test using a test concentration of 100 mg/L and one control group. The test concentration was analytically determined at the start and end of the experiment. The geometric mean test item concentration was 94.2 mg/L. As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on the nominal test item concentration.

Seven fish were used in each of the test and control groups. There were no deaths observed in either group during the study. No sub-lethal effects were observed in either group and no differences in body length and weight were observed between the groups.

All validity criteria were met during the study.

Under the conditions of this study the 96-h toxicity to Danio rerio was > 100 mg/L.

Acute toxicity to aquatic invertebrates: Acute toxicity of the test item on Daphnia magna was assessed in an acute immobilisation test performed according to OECD TG 202 over an exposure period of 48 hours in a static test system (Sipos, 2020).

Because no toxic response was observed during the preliminary range-finding test, a Limit Test was carried out using only one test concentration (100.0 mg a.i./L) and one control group in the definitive test.

The test concentration was analytically determined at the start and at the end of the experiment. Measured concentration was 98.53 mg a.i./L at the start and 105.27 mg a.i./L at the end of the experiment. As the measured concentration deviated not more than 20 per cent from the nominal, biological results are based on the nominal test item concentration.

Twenty animals, divided into four groups (glass beaker) of five animals each were used at the test concentration and for the control.

All validity criteria were met during this study.

Under the conditions of this Daphnia magna acute immobilisation study the observed endpoints for the effect of the test item were the following:

The 24h and 48h EC50 value: > 100.0 mg a.i./L (nominal)

The 48h EC100 value: > 100.0 mg a.i./L (nominal)

The 48h No-Observed Effect Concentration (NOEC): 100.0 mg a.i./L (nominal)

The 48h Lowest Observed Effect Concentration (LOEC): > 100.0 mg a.i./L (nominal)

Toxicity to aquatic algae: The toxicity of the test item to aquatic algae was assessed in a study performed according to OECD TG 201 over a period of 72 hours in a static test system using Pseudokirchneriella subcapitata (Citoxlab 2013).

Test concentrations used during the main study were nominal concentrations of test item used in the main experiment were 6.25, 12.5, 25, 50 and 100 mg/L (measured geometric mean test item concentrations 5.9, 11.8, 24.7, 48.8 and 93.8 mg/L, respectively). As the measured concentrations deviated not more than 20 per cent from the nominal in all cases, biological results are based on the nominal test item concentration.

Three replicates were used per test concentration and six replicates for the controls.

All validity criteria were met therefore the study can be considered as valid.

The results of the statistical evaluation (based on Bonferroni t-Test; a=0.05) show that the 0-72 h average specific growth rate was statistically significantly different from the untreated control value at the concentration range of 12.5 – 100 mg/L (nominal), correspondingly the NOEC was determined as 6.25 mg/L (nominal). The 72 h ErC50 value was determined as higher than100 mg/L(nominal).

The results of the statistical evaluation (based on Bonferroni t-Test; a=0.05) show that the 0-72 h areas were statistically significantly different from the untreated control value at the examined concentration range of 50 – 100 mg/L (nominal), correspondingly NOEC was determined as 25 mg/L (nominal). The 72 h EbC50 value was determined as higher than 100 mg/L(nominal).

The results of the statistical evaluation (based on Bonferroni t-Test; a=0.05) show that the 0-72 h yield was statistically significantly different from the untreated control value at the tested concentration range of 12.5 –100 mg/L (nominal), correspondingly the NOEC was determined as 6.25 mg/L (nominal).The 72 h EyC50 value was determined as higher than 100 mg/L(nominal).